Protein Info for Shewana3_1931 in Shewanella sp. ANA-3

Annotation: alpha-glucosidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 signal peptide" amino acids 1 to 47 (47 residues), see Phobius details PF14508: GH97_N" amino acids 51 to 300 (250 residues), 240.4 bits, see alignment E=2.7e-75 PF10566: Glyco_hydro_97" amino acids 318 to 505 (188 residues), 216.7 bits, see alignment E=2.6e-68 PF14509: GH97_C" amino acids 600 to 701 (102 residues), 115.1 bits, see alignment E=2.1e-37

Best Hits

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 100% identity to shn:Shewana3_1931)

Predicted SEED Role

"Alpha-glucosidase (EC 3.2.1.20)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KWJ3 at UniProt or InterPro

Protein Sequence (703 amino acids)

>Shewana3_1931 alpha-glucosidase (RefSeq) (Shewanella sp. ANA-3)
MFSNPLTHVRHINSPKRHSRHWAASLFTPSLLALSIGSLLCTSATAQTLNLSSPDKQIVL
SLSDDTGRPEYQIHFHGKSIIEPSRLGLVFASLGELGQGLRIKSSHSASADEQWQQPWGE
QEWIKDQHNQLSVVLTDGKIDLNLEFKAFDDGIGFRYHLAAQHALPNNTPLAITDELTEF
NIGQSDKATAWWIPSRGWNRYEYLYRTSALNQVDRAHTPFTFKLVDGTHLSIHEAALVDF
ASMTLDQQRDGKLKADLTPWSDGIRVKTQAGFYTPWRTIQIADKATGLLNSHLILNLNEP
NKLGDVSWVKPGKYVGIWWGMHLNENTWGSGPTHGATTSETERYMDFAAKYQFDGVLVEG
WNQGWDGDWFHNGDQFSFTKAYDDFDLPAITAYGAKKGVRLIGHHETAGSVTHYRNQMDD
AFALYEKHGVTQVKTGYVADGGQIKRIDENGITRHEWHDGQFMVGEYLHSVTEAAKHHIS
INSHEPIKDTGLRRTYPNWLAREGARGQEYNAWGSPPNNPEHTAMLPFTRMLAGPMDFTP
GIFDLAPKGLDAENRVQTTLMKQLALYVVLYSPIQMAADLPRNYQKHLDAFQFIRDVPTD
WYQSIALAGEVGDYVVFARQAKDQQDWYLGALTDENTREVKVKLDFLDPNKRYQAQIYRD
GAKADWKTNPYDYVIETKEVNAKDSLTLQLASSGGTAIRFKAL