Protein Info for Shewana3_1879 in Shewanella sp. ANA-3
Annotation: nickel-dependent hydrogenase, large subunit (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to MBHL_WOLSU: Quinone-reactive Ni/Fe-hydrogenase large chain (hydB) from Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)
KEGG orthology group: K05922, quinone-reactive Ni/Fe-hydrogenase large subunit [EC: 1.12.5.1] (inferred from 100% identity to shn:Shewana3_1879)Predicted SEED Role
"Uptake hydrogenase large subunit (EC 1.12.99.6)" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase (EC 1.12.99.6)
MetaCyc Pathways
- hydrogen to fumarate electron transfer (2/2 steps found)
- hydrogen oxidation I (aerobic) (1/1 steps found)
- hydrogen to dimethyl sulfoxide electron transfer (1/2 steps found)
- hydrogen to trimethylamine N-oxide electron transfer (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.12.5.1
Use Curated BLAST to search for 1.12.5.1 or 1.12.99.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0KWE1 at UniProt or InterPro
Protein Sequence (567 amino acids)
>Shewana3_1879 nickel-dependent hydrogenase, large subunit (RefSeq) (Shewanella sp. ANA-3) MSKRVVIDPITRIEGHLRIEVEVDENNVINKAWSSSTLWRGIEVILKGRTPMDVGLIVQR ICGVCTYSHYRCGTEAVENALGIQIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV VSALSADPAKAAQVALKYTDKPIAAGEGELRAVQERVKGFVETGKLGPFANAYWGNGTYK FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKQPHPQSLVVGGVTSVRDMLSPARLQEW KQKHAIVTDFILRAYQADIVMAAEAFGGEASVLGGVNVKNFMATNDFVLANGEYLFDQGV IMKGDLAGVSDINPELIAEDVSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLD GDGKYSWVKSPRYQGEPVEVGPLSCLLVSYARGNKVVVDAVNALLARTGLPVEALFTTLG RTAARMLQTVIVAQEGLKTFDALLTNIQSDETTYVKPHIDPSREYVGHAMIEAPRGMLSH WIRIKNGVIENYQAVVPTTWNAGPVDANGKIGPYEASLIGLKLEDPTKPLEVIRIIHSFD PCMACSVHVMDFKGQALSEFRVSPNGQ