Protein Info for Shewana3_1760 in Shewanella sp. ANA-3

Name: metG
Annotation: methionyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 676 TIGR00398: methionine--tRNA ligase" amino acids 8 to 534 (527 residues), 629.5 bits, see alignment E=4.9e-193 PF09334: tRNA-synt_1g" amino acids 8 to 396 (389 residues), 543.7 bits, see alignment E=6.9e-167 PF00133: tRNA-synt_1" amino acids 9 to 79 (71 residues), 33.7 bits, see alignment E=5.4e-12 PF19303: Anticodon_3" amino acids 412 to 525 (114 residues), 35.8 bits, see alignment E=2.2e-12 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 551 to 676 (126 residues), 147.1 bits, see alignment E=2.8e-47 PF01588: tRNA_bind" amino acids 581 to 673 (93 residues), 91.5 bits, see alignment E=8.9e-30

Best Hits

Swiss-Prot: 100% identical to SYM_SHESA: Methionine--tRNA ligase (metG) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to shn:Shewana3_1760)

MetaCyc: 68% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KW24 at UniProt or InterPro

Protein Sequence (676 amino acids)

>Shewana3_1760 methionyl-tRNA synthetase (RefSeq) (Shewanella sp. ANA-3)
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIM
LKAQQLGMAPEEMIAQVNKEHQQDFADFNIAFDNYHSTHSEENRVLASDIYLKLRANGYI
KSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDACGATYSPTELINPKSAVSG
ATPVMKDTEHFFFDLPAFEDMLKEWTRSGALQTEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKHPELSFDEFWAKDSKAEVYHFIGKDIVYF
HSLFWPAMLHGSGYRQPNSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
AKLSSRIDDLDLNLEDFVQRVNSDLVGKLVNLASRTAGFITKRFDGKLAKIADTTLTDAF
LAKQEQIAEFYETREYGKAMREIMALADIANGFVADAAPWQLVKQDDQQETAHQVCSNAL
NLFRILVTYLKPVLPRLALDVEAFFQQTLTWDALNQDMAGHEIAPFKAMMQRVELEKVNA
MVADSKENLQATSEPEAPKGPLATDPISDTINFEDFAKIDLRIARIVKAEHVAEADKLLK
LQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRKMRFGMSEGMVLAAGPGGSDL
WILEPHEGAQPGMRVK