Protein Info for Shewana3_1675 in Shewanella sp. ANA-3
Annotation: AMP-dependent synthetase and ligase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to Y3568_PSEAE: Uncharacterized protein PA3568 (PA3568) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 100% identity to shn:Shewana3_1675)MetaCyc: 59% identical to propionate--CoA ligase (Ruegeria pomeroyi DSS-3)
Propionate--CoA ligase. [EC: 6.2.1.17]
Predicted SEED Role
"Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 6.2.1.16)
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- superpathway of fatty acid biosynthesis II (plant) (37/43 steps found)
- 2-methylcitrate cycle I (5/5 steps found)
- superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
- 2-methylcitrate cycle II (5/6 steps found)
- palmitate biosynthesis III (21/29 steps found)
- acrylate degradation II (2/3 steps found)
- β-alanine biosynthesis II (4/6 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- 2-deoxy-D-ribose degradation II (3/8 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- stearate biosynthesis I (animals) (1/6 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.16
Use Curated BLAST to search for 6.2.1.16 or 6.2.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0KVT9 at UniProt or InterPro
Protein Sequence (640 amino acids)
>Shewana3_1675 AMP-dependent synthetase and ligase (RefSeq) (Shewanella sp. ANA-3) MMGGLMTDAGQQLHQTSIANKQAFWQQAAKALDWVTPSKQILDESEAPFYHWFSDGELNT CYNAVDRHVLAGRGEQIAIHYVSPVTETEYSITYRELQAQVARLAGYLQSVGVAKGDRVV IYMPMVPETAYAMLACARIGAIHSVVFGGFAANELATRINDAKPKLVMSASCGIEPSGVV PYKPLLDKALNEAVHKVEHCLILNRPQYEAQMQAGRDKDWQTALCTSDSADCVTVKATDP LYVLYTSGTTGQPKGVVRDNGGHAVALAWSMANIYDIAQGDVFWAASDVGWVVGHSYIVY GPLLVGATTLLYEGKPVGTPDPGAFWRTIAKYRVKSFFTAPTAIRAIKREDPDGEFILGV DLSCLKNVFLAGERCDPDTLHWAEAKLHKPVIDHWWQTETGWPVAANLMGVAPIAVKAGS PGRPVPGYEVDVVDELGAKVAANVSGNVVIKLPLPPGTLTTLWQNNKRYQDSYLSMYPGY YLTGDAGYMDEDGYLYIMSRIDDIINVAGHRLSTGRFEEVLCQHPDVAEAAVIGVDDKLK GQVPLGLVVLKKGVTITDEELHKQLIALVRQEIGPVASFRLVSAIQKLPKTRSGKILRGT MRKIADNQQYTAPATIEDPQTLDLVRTTLTRMGYADALVG