Protein Info for Shewana3_1577 in Shewanella sp. ANA-3

Annotation: exonuclease, RNase T and DNA polymerase III (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 PF00929: RNase_T" amino acids 43 to 204 (162 residues), 61 bits, see alignment E=1.1e-20

Best Hits

KEGG orthology group: K02342, DNA polymerase III subunit epsilon [EC: 2.7.7.7] (inferred from 100% identity to shn:Shewana3_1577)

Predicted SEED Role

"DNA polymerase III epsilon subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KVJ2 at UniProt or InterPro

Protein Sequence (228 amino acids)

>Shewana3_1577 exonuclease, RNase T and DNA polymerase III (RefSeq) (Shewanella sp. ANA-3)
MGPWWVNVKLRWRALRCQHRLFKDYYQSLVPLLELPISRAPLIAMDLEMTGLNPLQDQIL
SIGLVPIENGAIPLMGAQQKLVQIQGSVGQSATIHGILDNHLTEAVTIEEAMAWFVAQTR
GHVLVAHHSPLDCRFLQQDILSIYHQAIVLPAIDTLLLEKHRLLREHSVLKEGSLRLGAC
RERYGLPIYGAHSALTDALACGELLLAQVASMGGADELKVGELLAYSR