Protein Info for Shewana3_1569 in Shewanella sp. ANA-3

Annotation: DNA ligase, NAD-dependent (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 PF01653: DNA_ligase_aden" amino acids 27 to 338 (312 residues), 434.8 bits, see alignment E=7.8e-134 TIGR00575: DNA ligase, NAD-dependent" amino acids 32 to 683 (652 residues), 874.3 bits, see alignment E=2.8e-267 PF03120: DNA_ligase_OB" amino acids 342 to 418 (77 residues), 115 bits, see alignment E=4.7e-37 PF03119: DNA_ligase_ZBD" amino acids 427 to 454 (28 residues), 42.1 bits, see alignment (E = 2.5e-14) PF14520: HHH_5" amino acids 467 to 520 (54 residues), 18.6 bits, see alignment 8.7e-07 amino acids 531 to 578 (48 residues), 24.9 bits, see alignment 9.4e-09 PF12826: HHH_2" amino acids 530 to 593 (64 residues), 85.5 bits, see alignment E=9.1e-28 PF00533: BRCT" amino acids 613 to 686 (74 residues), 53.6 bits, see alignment E=8.6e-18 PF12738: PTCB-BRCT" amino acids 629 to 678 (50 residues), 25.4 bits, see alignment 4.7e-09

Best Hits

Swiss-Prot: 100% identical to DNLJ_SHESA: DNA ligase (ligA) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to shn:Shewana3_1569)

MetaCyc: 62% identical to DNA ligase (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KVI4 at UniProt or InterPro

Protein Sequence (691 amino acids)

>Shewana3_1569 DNA ligase, NAD-dependent (RefSeq) (Shewanella sp. ANA-3)
MNMQDIQLDKRLSELLSQAVTPHNAQPIMQALCQSLNEHNIRYYVDDAPSITDSEYDRLM
QRLKQLEAEYPQFVAADSPTQRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVS
DRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGD
NYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRADL
PFEIDGVVMKVNDIAQQQTLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPV
ARLKPVFVGGVTVSNATLHNADEIARLGVMVGDTVIIRRAGDVIPQIVAIVPERRPEDAK
AIAFPQHCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLTASMMTMLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREV
GETTAANLATHFGSLEALRVATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAG
VNWPAIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQALGAKVAGSVSKNTDCLVAG
EAAGSKLAKAQELGVKVIGEDELLALLAANS