Protein Info for Shewana3_1555 in Shewanella sp. ANA-3

Annotation: formate acetyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 11 to 759 (749 residues), 1353.5 bits, E=0
• PF02901: PFL-like
  • amino acids 23 to 614 (592 residues), 718.9 bits, E=7.2e-220
• PF01228: Gly_radical
  • amino acids 630 to 741 (112 residues), 119.6 bits, E=8.4e-39

Best Hits

Swiss-Prot: 82% identical to PFLB_ECOLI: Formate acetyltransferase 1 (pflB) from Escherichia coli (strain K12)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 82% identity to ecl:EcolC_2693)

MetaCyc: 75% identical to 2-oxobutanoate formate-lyase/pyruvate formate-lyase 4 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• mixed acid fermentation (15/16 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• reductive monocarboxylic acid cycle (1/2 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.54

Sequence Analysis Tools

See A0KVH0 at UniProt or InterPro

Protein Sequence (760 amino acids)

>Shewana3_1555 formate acetyltransferase (RefSeq) (Shewanella sp. ANA-3)
MTDKTELFSNAWEGFTPGDWKSEVNVRDFIQQNYAPYEGDESFLAGATEATTQLWDKVME
GIKQENRTHAPVDFDTKMVSTITSHDAGYINKDLETIVGLQTDAPLKRAMLPNGGIRMVE
GSCAAYNRELDADVKYIYSELRKTHNQGVFDVYTPEIMACRKSGVLTGLPDAYGRGRIIG
DYRRVALYGIDFLMKDKFAQFSSLQAQFEAGEDLSNVIQLREEIAEQHRALGQMKTMAAK
YGFDISRPAANAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTITEQ
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNT
LYTMGPSPEPNITVLWSDKLPVGFKKYCAKVSIDTSSIQYENDDLMRPDFQSDDYAIACC
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQIAPKAAPITDEVLNFDDVMNRL
DGLMDWLATQYVTALNSIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
KYAQVKPVRDENGIAVDFEISGDYPKFGNNDPRVDDIACDLVERFMSKIRDRKMYRNAIP
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
QDGISYTFSIVPNALGKDEDGRRTNLAALMDGYFAHNEGHEGGQHLNVNVMNREMLEDAV
VNPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL