Protein Info for Shewana3_1483 in Shewanella sp. ANA-3

Annotation: amidophosphoribosyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01134: amidophosphoribosyltransferase
  • amino acids 2 to 463 (462 residues), 535 bits, E=9.9e-165
• PF13230: GATase_4
  • amino acids 62 to 182 (121 residues), 23.3 bits, E=5.6e-09
• PF13522: GATase_6
  • amino acids 64 to 206 (143 residues), 60.9 bits, E=2.8e-20
• PF13537: GATase_7
  • amino acids 80 to 212 (133 residues), 55.4 bits, E=1.3e-18
• PF00156: Pribosyltran
  • amino acids 279 to 392 (114 residues), 32.2 bits, E=1.3e-11

Best Hits

Swiss-Prot: 73% identical to PUR1_SHIFL: Amidophosphoribosyltransferase (purF) from Shigella flexneri

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 100% identity to shm:Shewmr7_1495)

MetaCyc: 73% identical to amidophosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Amidophosphoribosyltransferase. [EC: 2.4.2.14]

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

See A0KV98 at UniProt or InterPro

Protein Sequence (504 amino acids)

>Shewana3_1483 amidophosphoribosyltransferase (RefSeq) (Shewanella sp. ANA-3)
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHM
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
VNTTSDSEVLLNLLADELQKTTSLMLTPDEVFDTIAKVHEQARGAYAVVAMIIGQGLVAF
RDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYISLDGQLYTRQ
CAKEPSYSPCIFEFVYFARPDSTIDNVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
DEIAKLIGADGIIFQNLPDLVEAVRMENPEIKRFETSVFDGHYITNDVDQSYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP