Protein Info for Shewana3_1441 in Shewanella sp. ANA-3

Annotation: siroheme synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 186 (183 residues), 177.1 bits, see alignment E=1.8e-56 PF13241: NAD_binding_7" amino acids 6 to 115 (110 residues), 90.9 bits, see alignment E=1.1e-29 PF14824: Sirohm_synth_M" amino acids 119 to 145 (27 residues), 28.4 bits, see alignment (E = 1.4e-10) PF10414: CysG_dimeriser" amino acids 151 to 184 (34 residues), 34 bits, see alignment 3e-12

Best Hits

KEGG orthology group: K02304, precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 4.99.1.4] (inferred from 100% identity to shn:Shewana3_1441)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.1.76, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.76 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KV56 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Shewana3_1441 siroheme synthase (RefSeq) (Shewanella sp. ANA-3)
MQYFPLFIDTVNLNVLLVGAGDVASRKLALLTRTEANIHVIAPEVNPEVKAYADAGRILL
SERPVVQADIQNYDLIYLATANDKLNAELATLATERGIWVNAVDNPAYCRFITPSIVDRG
RLVVAISTAGAAPVFARTIRARLETSLPQSLKPLFDFVADKRLEVQQRLTKTATRRLFWE
RFFDTNGDRFDARTPEHYQNAFNHIVSRGEILLLDDATPVELLPLAAMPLLQRLDWIYSE
AGLAEDLAELVRRDANRAALPALSDISREYEQGSRMLLVADTQKIAQLKAHFPVAKHLRP
GAI