Protein Info for Shewana3_1422 in Shewanella sp. ANA-3

Annotation: 4a-hydroxytetrahydrobiopterin dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 PF00270: DEAD" amino acids 33 to 205 (173 residues), 162 bits, see alignment E=1.7e-51 PF04851: ResIII" amino acids 48 to 201 (154 residues), 33.8 bits, see alignment E=4.9e-12 PF00271: Helicase_C" amino acids 240 to 348 (109 residues), 105.4 bits, see alignment E=3e-34

Best Hits

Swiss-Prot: 47% identical to RHLE_ECOLI: ATP-dependent RNA helicase RhlE (rhlE) from Escherichia coli (strain K12)

KEGG orthology group: K01724, 4a-hydroxytetrahydrobiopterin dehydratase [EC: 4.2.1.96] (inferred from 100% identity to shn:Shewana3_1422)

MetaCyc: 47% identical to ATP-dependent RNA helicase RhlE (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase VF1437" in subsystem ATP-dependent RNA helicases, bacterial

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.96

Use Curated BLAST to search for 4.2.1.96 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KV37 at UniProt or InterPro

Protein Sequence (427 amino acids)

>Shewana3_1422 4a-hydroxytetrahydrobiopterin dehydratase (RefSeq) (Shewanella sp. ANA-3)
MTQASSSVASFAELGIIAPLCNRLSELTYAAPTPIQAATIPAVLSGRDVLAGANTGSGKT
AAFAVPLLQRLFEAKTAEKSAGQVRCLVLVPTRELAQQVADSFLSYASHFNGQLKIVAAF
GGVSVNLQMQSLRAGADVLVATPGRLLDLLASNALKLNRVSALVLDEADRMLSLGFTDEL
NQVLEALPSKKQTLLYSATFPEEVRALTAKLLHQPLEYHLQSEQESTIEQRVITVNREQK
TALLAHLIKQHQWSQALIFVSAKNTCNHLAQKLSKRGISAEVFHGDKAQGARTRVLDGFK
SGEISVLIATDIAARGIDIDKLPVVINFDLPRSPADYMHRIGRSGRAGEAGLAVTLISHE
EYHHFGVIEKKNKIKLVREQIPGFEANAEMPLEVLAQEKPQAKPEGTGKKKRKQLPAANL
EFWGKKS