Protein Info for Shewana3_1289 in Shewanella sp. ANA-3

Annotation: conjugal transfer protein TrbF (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 transmembrane" amino acids 43 to 61 (19 residues), see Phobius details PF04335: VirB8" amino acids 23 to 225 (203 residues), 228.3 bits, see alignment E=5e-72

Best Hits

KEGG orthology group: K03200, type IV secretion system protein VirB5 (inferred from 100% identity to shn:Shewana3_1289)

Predicted SEED Role

"Conjugative transfer protein TrbF" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KUQ4 at UniProt or InterPro

Protein Sequence (234 amino acids)

>Shewana3_1289 conjugal transfer protein TrbF (RefSeq) (Shewanella sp. ANA-3)
MRFKRPQVRYADTPQPATPYQAAAQVWDERIGSARVQAKNWRLMAFGCLVLALLMAGGLV
WRSAQSIVTPYVIEVDQAGQVRAVGEAATPYRPADAQIAHHLARFVSLVRSLSIDPIVVR
QNWLDAYDYTTDRGAAVLNDYARTNDPFARIGKESVTVQITSVVRASDTSFNVRWTEQRF
VNGAPAGTERWNAVLSTVLQTPRTEQRLRKNPLGIYVNGLSWSRELDSSEGAKP