Protein Info for Shewana3_1035 in Shewanella sp. ANA-3

Annotation: polynucleotide phosphorylase/polyadenylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 9 to 690 (682 residues), 1077.3 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 143 (130 residues), 109.7 bits, see alignment E=4.4e-35 amino acids 323 to 455 (133 residues), 94.4 bits, see alignment E=2.3e-30 PF03725: RNase_PH_C" amino acids 147 to 201 (55 residues), 43.4 bits, see alignment 7.9e-15 amino acids 460 to 526 (67 residues), 25.9 bits, see alignment E=2.4e-09 PF03726: PNPase" amino acids 241 to 319 (79 residues), 75.2 bits, see alignment E=1.4e-24 PF00013: KH_1" amino acids 555 to 613 (59 residues), 40.3 bits, see alignment 6.6e-14 PF00575: S1" amino acids 618 to 689 (72 residues), 67.8 bits, see alignment E=2.6e-22

Best Hits

Swiss-Prot: 100% identical to PNP_SHESA: Polyribonucleotide nucleotidyltransferase (pnp) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to shn:Shewana3_1035)

MetaCyc: 73% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KU01 at UniProt or InterPro

Protein Sequence (699 amino acids)

>Shewana3_1035 polynucleotide phosphorylase/polyadenylase (RefSeq) (Shewanella sp. ANA-3)
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVD
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVTQLESSQLNLVVAG
TESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEDGLAQAYQIQVKQDRYAQVAVVKAAAKEALLAANPDVDLREVDGLLGSLE
KKVVRGRIIRGEPRIDGREPDMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTAEEF
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
GDATREAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
VSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE