Protein Info for Shewana3_1034 in Shewanella sp. ANA-3

Annotation: diguanylate cyclase/phosphodiesterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 transmembrane" amino acids 9 to 28 (20 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 235 to 398 (164 residues), 139.9 bits, see alignment E=3.3e-45 PF00990: GGDEF" amino acids 239 to 396 (158 residues), 163.1 bits, see alignment E=4.7e-52 PF00563: EAL" amino acids 417 to 647 (231 residues), 224.3 bits, see alignment E=1.5e-70

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_1034)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KU00 at UniProt or InterPro

Protein Sequence (674 amino acids)

>Shewana3_1034 diguanylate cyclase/phosphodiesterase (RefSeq) (Shewanella sp. ANA-3)
METNKSRRFYSAFFAIAIFIVEIALVVSTKQQLTTTQQELLDSVEQPAANAVWALDDNLA
RQTLEGAIKVEHVGSAVIELDDGSMFVSVSNNKKNNSQTFISLSNKLFDDLKEISRPLYR
PFYFEGTQKQQLIGTLTIFYDTQELTNTLFSQLRFSFFATLARALLLTLVLSVVFHRFLT
QPIARISEAIDKISPDSPDENLLPMSNAHKDDELGLVTSKFNQILIQFSQTQSKLRKMAT
RDPLTGLPNRTLLLETIAVTIQRSRVHKSSFALMFIDLDRFKNINDSLGHALGDQFLARI
ARILERVVGDKGSVSRLGGDEFVILADSVHSPDQAADFVDKLLVQLNVPIQLNEHAIHPA
ASIGISIYPEDGNSAEDLIRHADIAMYSAKAAGSNQWAFFKQQMTERAAVRLRTEASLHD
ALKNNEFLLYFQPKLDLQTGKITACEALIRWQKDGRLISPISFIPVAEETGIIIPIGRWV
IEQSCRVLRDWQKKYNYAIPIAINVASQQFADASLVSDIKQMALRYQIQPELLEIEITET
SLMNDIELAINKLEQLKSAGFGIAVDDFGTGYSSLSYLRHLPITTMKIDRCFVSDLPGDS
AIASTILMLGKQLNLTIVAEGIENEQQLDWLKRNQCQIGQGFYFSQPLPQAEFEALYIAN
QTATISNIDGFRGA