Protein Info for Shewana3_0951 in Shewanella sp. ANA-3

Annotation: dihydrofolate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 PF00883: Peptidase_M17" amino acids 175 to 490 (316 residues), 200.9 bits, see alignment E=1.6e-63

Best Hits

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to shn:Shewana3_0951)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KTR7 at UniProt or InterPro

Protein Sequence (512 amino acids)

>Shewana3_0951 dihydrofolate reductase (RefSeq) (Shewanella sp. ANA-3)
MFQVNFVDVQAENAIFDGEGWDAVVVVTPDLVSIGIDEISLLAEHGAKVDKRVGKSPTLL
FAPGLAGGRLIIAPVTQVADDYADVRIYGDAARAAIAIAKDAGAKRPLLYVVPSATPKFG
FASEVAALACGQELWQTLELREATGLTPAFEAIGLLSLSSEKSHLLNALEAGRVLARDLC
GTEPERMSAKAFAEYCLQAFKGSVIKTAVVEDRDILERDYPLLSAVARSSFAVGRHQPRV
VKLEYQPEGEITRTFLFAGKGVVYDTGGADLKVGGAMAGMSRDKGGASAVAGLFKTLSML
KPKGIRVIAELGLVRNSIGSEAFVTDEIITSHAGVRVRIGNTDAEGRLVLADLLSHLRIK
AVSAVQPELFSVATLTGHVVRCYGSYPAAVENAVASGAQCAQTLALQASQWGEPFEVSTL
RRDDFAKIREVSGAADILSSNNAPSSVTARGHQYPAAFLLKASGLFDHGINAAIPLAYTH
LDIAGSATEGDPLYGKPTASPLVGLYQYLINR