Protein Info for Shewana3_0854 in Shewanella sp. ANA-3

Name: pntA
Annotation: NAD(P) transhydrogenase subunit alpha (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 transmembrane" amino acids 166 to 189 (24 residues), see Phobius details amino acids 398 to 417 (20 residues), see Phobius details amino acids 423 to 441 (19 residues), see Phobius details amino acids 450 to 469 (20 residues), see Phobius details amino acids 475 to 497 (23 residues), see Phobius details TIGR00561: NAD(P)(+) transhydrogenase (AB-specific), alpha subunit" amino acids 2 to 507 (506 residues), 879.5 bits, see alignment E=3.6e-269 PF05222: AlaDh_PNT_N" amino acids 4 to 134 (131 residues), 162.2 bits, see alignment E=1.2e-51 PF01262: AlaDh_PNT_C" amino acids 139 to 367 (229 residues), 244.9 bits, see alignment E=9.4e-77 PF12769: PNTB_4TM" amino acids 424 to 507 (84 residues), 126 bits, see alignment E=9.9e-41

Best Hits

Swiss-Prot: 70% identical to PNTA_HAEIN: NAD(P) transhydrogenase subunit alpha (pntA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 99% identity to she:Shewmr4_3081)

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KTH3 at UniProt or InterPro

Protein Sequence (508 amino acids)

>Shewana3_0854 NAD(P) transhydrogenase subunit alpha (RefSeq) (Shewanella sp. ANA-3)
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVVEANAGLLSSFDDAAFEAAGAKITAE
VWQADLIFKVNAPTDAEIAQIKEGATLISFIWPAQNAELVEKLSKRNINVMAMDMVPRIS
RAQSLDALSSMANIGGYRAVVEAAHHFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
TAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFTNEDGSSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKPGSVIVDMAAQAGGNCEYTQPSELF
VTENGVKVIGFTDLPGRLPAQSSQLYGTNLVNLMKLMCKEKNGVASINFDDVVMRNMTVV
KAGEVTFPPPAISVSAAPAKPAAKVEAKAAEAKKPSKLKYILAALGAAGFAAVASVAPAE
FLSHFTVFVLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD