Protein Info for Shewana3_0770 in Shewanella sp. ANA-3

Annotation: HemK family modification methylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 TIGR00536: methyltransferase, HemK family" amino acids 6 to 280 (275 residues), 281.3 bits, see alignment E=7.5e-88 PF17827: PrmC_N" amino acids 9 to 78 (70 residues), 76.3 bits, see alignment E=1.2e-24 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 28 to 276 (249 residues), 291.3 bits, see alignment E=6.3e-91 PF05175: MTS" amino acids 100 to 197 (98 residues), 70.8 bits, see alignment E=6.3e-23 PF06325: PrmA" amino acids 108 to 188 (81 residues), 28.3 bits, see alignment E=6.8e-10 PF02384: N6_Mtase" amino acids 112 to 238 (127 residues), 25.9 bits, see alignment E=3.4e-09 PF13847: Methyltransf_31" amino acids 113 to 245 (133 residues), 60.9 bits, see alignment E=7e-20 PF03602: Cons_hypoth95" amino acids 114 to 195 (82 residues), 30.2 bits, see alignment E=1.9e-10 PF13649: Methyltransf_25" amino acids 118 to 188 (71 residues), 38.4 bits, see alignment E=9.5e-13 PF08241: Methyltransf_11" amino acids 119 to 188 (70 residues), 30 bits, see alignment E=3.8e-10 PF01170: UPF0020" amino acids 133 to 197 (65 residues), 23.8 bits, see alignment E=1.9e-08

Best Hits

Swiss-Prot: 96% identical to PRMC_SHEON: Release factor glutamine methyltransferase (prmC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 99% identity to shm:Shewmr7_0798)

MetaCyc: 52% identical to protein-(glutamine-N5) methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-14992 [EC: 2.1.1.297]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.297

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KT89 at UniProt or InterPro

Protein Sequence (286 amino acids)

>Shewana3_0770 HemK family modification methylase (RefSeq) (Shewanella sp. ANA-3)
MADQSSIAEALQWAYVQLAPTSESAHLDAEVFLLYCLNKNRAFLYTWPEKALTVEQWKRF
QQMVQRRQQGVPVAHIVGEREFWSLPFIVNDTTLIPRPDTEILVETALNLPLESNAKVLD
LGTGTGAIALALASERATWQITAVDKVEDAVALAKANRTNLKLEQVEILQSDWFSAIKAH
DFDLIVSNPPYIDEADEHLHQGDVRFEPQSALTAADEGFADLYYIAKTARDYLKPNGYIL
LEHGFEQAVKLREKLTELGYQNVATVRDFGSNDRCTMGKWMGLIGI