Protein Info for Shewana3_0750 in Shewanella sp. ANA-3

Annotation: malate dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 78 to 258 (181 residues), 258.5 bits, E=3.5e-81
• PF03949: Malic_M
  • amino acids 268 to 528 (261 residues), 350.4 bits, E=5.5e-109

Best Hits

Swiss-Prot: 100% identical to MAO1_SHESA: NAD-dependent malic enzyme (maeA) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K00027, malate dehydrogenase (oxaloacetate-decarboxylating) [EC: 1.1.1.38] (inferred from 100% identity to shn:Shewana3_0750)

MetaCyc: 68% identical to malate dehydrogenase (oxaloacetate-decarboxylating) (Escherichia coli K-12 substr. MG1655)
Malate dehydrogenase (oxaloacetate-decarboxylating). [EC: 1.1.1.38]

Predicted SEED Role

"NAD-dependent malic enzyme (EC 1.1.1.38)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.38)

MetaCyc Pathways

• gluconeogenesis I (13/13 steps found)
• mixed acid fermentation (15/16 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• malate/L-aspartate shuttle pathway (2/2 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• L-malate degradation II (1/1 steps found)
• chitin deacetylation (3/4 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
• anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• gluconeogenesis III (8/12 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• L-carnitine degradation II (1/3 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• methylgallate degradation (2/6 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• reductive TCA cycle II (6/12 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• methylaspartate cycle (9/19 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• syringate degradation (3/12 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.38

Sequence Analysis Tools

See A0KT69 at UniProt or InterPro

Protein Sequence (562 amino acids)

>Shewana3_0750 malate dehydrogenase (RefSeq) (Shewanella sp. ANA-3)
MDDNKRPLYLPFAGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQ
FRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNR
GLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCG
GISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLI
QFEDFAQKNAMPILERYKDRYCCFNDDIQGTAAVTVGSLLAACKAAGTELNKQRIAFLGA
GSAGCGIAEAIVAQMVSEGISDEQARSQVCMVDRWGLLLDNMPNLLPFQQKLAQKCADIC
NWNNFSDNISLLDVVNNAKPTVLIGVSGAPGLFTEEIVRAMHSHCERPIIFPLSNPTSRV
EATPKDILHWTSGQALVATGSPFEPVVVDGETYEIAQCNNSFIFPGIGLGVLASGARHVS
DAMLMASSRALAECSPLAINGSGPLLPKLEDIHSVSKHIAFAVGKVAIEQGLSLPASDEL
LMQSIEDNFWKPEYRRYKRTSF