Protein Info for Shewana3_0431 in Shewanella sp. ANA-3

Annotation: peptidase M13 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF05649: Peptidase_M13_N" amino acids 46 to 424 (379 residues), 407.7 bits, see alignment E=7.1e-126 PF01431: Peptidase_M13" amino acids 476 to 677 (202 residues), 250.7 bits, see alignment E=1e-78

Best Hits

KEGG orthology group: K01415, endothelin-converting enzyme [EC: 3.4.24.71] (inferred from 100% identity to shn:Shewana3_0431)

Predicted SEED Role

"Peptidase, M13 family"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.71

Use Curated BLAST to search for 3.4.24.71

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KSA5 at UniProt or InterPro

Protein Sequence (680 amino acids)

>Shewana3_0431 peptidase M13 (RefSeq) (Shewanella sp. ANA-3)
MKKISTLALGIALSLGLAACSSEPKTEVAAVSGIELQNFDASVRHQDDFYYSVNGKWLAN
TPIPADKSNYGAFSVLYDQSQDALKKIIDAAAAQKDAAPGSNNQKIGDFNASFMNTDLLE
SLGVTPLNPLLADISAVKNHSELPAVMGKLLTSGSGIPFGFYVNNDAKNSTQYAVYLSQS
GLTLPDRDYYLKDDAKFVANRQAMAKYVTDILTEAGYKNAKRAAKNVADIEMMIAKSQWS
RVESRDANKSYNKLSRAELQKLTGKFDINAFATSAGLGDKVTEVIVRQPSYLEKLGANFD
AFPVSAWQDYLTFHLVDSYAELLSKNFVDLNFAFKGKTLMGIEEQQPRWKKAVDGADQVI
GELVGEEYVKQYFKPEAKARMETMIKNLIKAFEVSINELEWMTPETKVAAQEKLSKFTYK
IGYPDKWKDYTALEIKPDELVGNYMRYANFEYQDMINKLGKPIDRTEWHMTPQTVNAYYS
PVGNEIVFPAAILQPPFFNMEADDAVNYGGIGAVIGHEISHGFDDQGAKYDGDGNLRDWW
SDKDREEFQKRGKQLSAQYSGYEALPGKFVNGDLTLGENIGDLGGLTVAARAYAMSLNGK
PAPVLDGLTGEQRFFVGWSQVWRRNYRDEELGRRLLTDPHSPSHYRAMGTPRNIEAFYKA
FELKESDKMYLKPEERVKIW