Protein Info for Shewana3_0374 in Shewanella sp. ANA-3

Annotation: ATP-dependent DNA helicase Rep (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 TIGR01074: ATP-dependent DNA helicase Rep" amino acids 2 to 668 (667 residues), 1110.7 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 3 to 266 (264 residues), 310.5 bits, see alignment E=3.9e-96 PF13245: AAA_19" amino acids 7 to 249 (243 residues), 88.2 bits, see alignment E=1.6e-28 PF13361: UvrD_C" amino acids 271 to 614 (344 residues), 276.3 bits, see alignment E=1.5e-85 PF13538: UvrD_C_2" amino acids 555 to 610 (56 residues), 34.4 bits, see alignment 5e-12

Best Hits

Swiss-Prot: 68% identical to REP_ECOLI: ATP-dependent DNA helicase Rep (rep) from Escherichia coli (strain K12)

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 100% identity to shn:Shewana3_0374)

MetaCyc: 68% identical to ATP-dependent DNA helicase Rep (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KS48 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Shewana3_0374 ATP-dependent DNA helicase Rep (RefSeq) (Shewanella sp. ANA-3)
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYNARNIAAVTFTNKAA
REMKERVAQSMGRKEARGLWISTFHTLGLEIVKREHKVLGLKPGFSLFDDQDTLALLKEL
TQKELQEDKDLLKALAGAISNWKGGLIIPDQARKLAKDEQQQLFAMLYQRYAQHMKAYNA
LDFDDLILLPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPKLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
SLFSELAYGEPMRVLFAANEEQEAERVVAEIIRHKFVGRTQFGDYAILYRGNHQSRLLER
ALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRVVNLPKRGIGPSTLER
LGNFANEKHISMFEAIFDPDLNHHLPPNAMSALYQFGRFIVDTGEHAERGEPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLSGDELDEPMTLPEVVNRLTLRDM
MERNSEDEGGDQVQLMTLHASKGLEFPYVFMVGVEERILPHQTSIDEDNVDEERRLAYVG
ITRAQRELWFTLCRERRQFGETMRCEPSRFLHELPQDDLIWENRKPQQTEQERMQTGRSH
ISNLRDMFKKQ