Protein Info for Shewana3_0335 in Shewanella sp. ANA-3

Annotation: histidine ammonia-lyase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00221: Lyase_aromatic
  • amino acids 22 to 485 (464 residues), 529.8 bits, E=2.8e-163

Best Hits

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to shn:Shewana3_0335)

Predicted SEED Role

"Putative histidine ammonia-lyase protein"

MetaCyc Pathways

• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)
• L-histidine degradation II (3/5 steps found)

KEGG Metabolic Maps

• Histidine metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

See A0KS09 at UniProt or InterPro

Protein Sequence (521 amino acids)

>Shewana3_0335 histidine ammonia-lyase (RefSeq) (Shewanella sp. ANA-3)
MSHAVTHTTTTEQPIEFGRQLLTLEQVVAVAKGAKVKLCDDADYQAYIQKGARFIDSLLH
EEGVVYGVTTGYGDSCTVNVSLDLVHELPLHLSRFHGCGLGEVLSVMQARAVMACRLNSL
AIGKSGVTYELLKRIETLLNLNIVPVIPEEGSVGASGDLTPLSYLAAALVGEREVIYNGE
RRATQEVYRELNITPHVLRPKEGLALMNGTAVMTALACLAFDRAQYLARLASRITAMASL
TLKGNSNHFDDILFAAKPHPGQNQIATWIREDLNHHVHPRNSDRLQDRYSIRCAPHIIGV
LQDALPFMRQFIETEVNSANDNPIVDGEGEHILHGGHFYGGHIAFAMDSLKNTVANIADL
IDRQMALVMDPKFNNGLPANLSGSTGARRAINHGFKAVQIGVSAWTAEALKHTMPASVFS
RSTECHNQDKVSMGTIAARDCMRVLQLTEQVAAAALLAMTQGIGLRITQNELDEASLTPS
LATTLAQVRADFEPLVEDRPLEAVLRQTVAKIQAGEWEVCR