Protein Info for Shewana3_0224 in Shewanella sp. ANA-3

Annotation: DNA-directed RNA polymerase subunit alpha (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 TIGR02027: DNA-directed RNA polymerase, alpha subunit" amino acids 24 to 315 (292 residues), 367 bits, see alignment E=3.4e-114 PF01193: RNA_pol_L" amino acids 29 to 227 (199 residues), 81.3 bits, see alignment E=5.2e-27 PF01000: RNA_pol_A_bac" amino acids 59 to 177 (119 residues), 125.7 bits, see alignment E=1.7e-40 PF03118: RNA_pol_A_CTD" amino acids 243 to 307 (65 residues), 84.2 bits, see alignment E=6.2e-28

Best Hits

Swiss-Prot: 100% identical to RPOA_SHESR: DNA-directed RNA polymerase subunit alpha (rpoA) from Shewanella sp. (strain MR-7)

KEGG orthology group: K03040, DNA-directed RNA polymerase subunit alpha [EC: 2.7.7.6] (inferred from 99% identity to sbl:Sbal_4145)

MetaCyc: 88% identical to RNA polymerase subunit alpha (Escherichia coli K-12 substr. MG1655)
DNA-directed RNA polymerase. [EC: 2.7.7.6]

Predicted SEED Role

"DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KRP9 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Shewana3_0224 DNA-directed RNA polymerase subunit alpha (RefSeq) (Shewanella sp. ANA-3)
MQGSVTEFLKPRLVDIEQVNSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSSKEGVQEDILEILLNLKGLAVTIEGKDEAMLTLSKSGAGPVIAADITHDGD
VTIVNPDHVICHLTGNNDISMRIRVERGRGYVPASARAQTEDDDRPIGRLLVDASFSPVA
RIAYNVEAARVEQRTDLDKLVIDMTTNGTIDPEEAIRRSATILAEQLDAFVELRDVTEPE
MKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLT
EIKDVLASRGLSLGMRLENWPPASLADDL