Protein Info for Shewana3_0153 in Shewanella sp. ANA-3

Annotation: general secretion pathway protein F (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 transmembrane" amino acids 171 to 194 (24 residues), see Phobius details amino acids 223 to 244 (22 residues), see Phobius details amino acids 325 to 339 (15 residues), see Phobius details amino acids 377 to 401 (25 residues), see Phobius details TIGR02120: type II secretion system protein F" amino acids 4 to 405 (402 residues), 577.9 bits, see alignment E=6.9e-178 PF00482: T2SSF" amino acids 73 to 195 (123 residues), 123.5 bits, see alignment E=2.6e-40 amino acids 275 to 397 (123 residues), 97.7 bits, see alignment E=2.5e-32

Best Hits

Swiss-Prot: 58% identical to GSPF_VIBCH: Type II secretion system protein F (epsF) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K02455, general secretion pathway protein F (inferred from 100% identity to shn:Shewana3_0153)

MetaCyc: 49% identical to type II secretion system protein GspF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"General secretion pathway protein F"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KRH8 at UniProt or InterPro

Protein Sequence (407 amino acids)

>Shewana3_0153 general secretion pathway protein F (RefSeq) (Shewanella sp. ANA-3)
MPAFEYKALDAKGKQLKGVIEADTARHARSQLRDQRMMPLEILPVTEKEAKSKGTGFSPF
KRGISVAELALITRQIATLVAAGLPIEECLKAVGQQCEKARLASMIMAVRSRVVEGYSLA
DSLAEFPHIFDDLYRAMVASGEKSGHLEVVLNRLADYTERRQQLKSKLQQAMIYPIMLTI
VAIGVVSVLLAAVVPKVVGQFEHMGAELPATTRFLIAASDFVQHYGLFVILAIGILLVVF
QRLLKSPIFKMKYHTFLLKMPVIGRVSKGLNTARFARTLSILSASSVPLLDGMRIASEVL
QNVRVRAAVDDATARVREGTSLGAALTNTKLFPAMMLYMIASGEKSGQLEEMLERAADNQ
DREFESNVTLALGVFEPMLVVSMAGVVLFIVMAILQPILALNNLISG