Protein Info for Shewana3_4155 in Shewanella sp. ANA-3

Annotation: RND family efflux transporter MFP subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF25973: BSH_CzcB" amino acids 84 to 145 (62 residues), 30.1 bits, see alignment E=5.5e-11 PF25954: Beta-barrel_RND_2" amino acids 159 to 235 (77 residues), 34.8 bits, see alignment E=2.4e-12 PF25975: CzcB_C" amino acids 242 to 301 (60 residues), 83.1 bits, see alignment E=1.6e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_4155)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L2X8 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Shewana3_4155 RND family efflux transporter MFP subunit (RefSeq) (Shewanella sp. ANA-3)
MKSNTQLKKSFIRLITCCFAITLLSGQTFAETGHGEEEEHGEEGHVELTQEQIKHAGITL
ATVTSGSIRDVLPVYGVVATNAERVQSVTARFDGVIREVKKSVGDPVRKGEPLVTVEANE
SLKTYSIVSALNGAVTQRNANAGEHTTDIPLLVVQDFSTVWVELSVFPKDVAQVELGQRV
RILSLDSTHIAEGKIIYIAPLGQTGNQAIMARALIDNPNGIWKPGLFVNAQITRAEIAAP
MVIRNEALQIVEDNPVVFVRGEEGFEARTVTLGRTDGELTEVVAGLSADEVYVSKNSFIL
KAELGKGEVGDDD