Protein Info for Sama_3642 in Shewanella amazonensis SB2B

Annotation: UDP-N-acetylglucosamine diphosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 452 (449 residues), 632.7 bits, E=1.7e-194
• PF01128: IspD
  • amino acids 4 to 126 (123 residues), 33.7 bits, E=8.1e-12
• PF00483: NTP_transferase
  • amino acids 5 to 202 (198 residues), 57.1 bits, E=5.2e-19
• PF12804: NTP_transf_3
  • amino acids 5 to 128 (124 residues), 81.1 bits, E=2.7e-26
• PF00132: Hexapep
  • amino acids 392 to 426 (35 residues), 28.3 bits, 2.6e-10
• PF14602: Hexapep_2
  • amino acids 392 to 425 (34 residues), 26.1 bits, 1.5e-09

Best Hits

Swiss-Prot: 100% identical to GLMU_SHEAM: Bifunctional protein GlmU (glmU) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to saz:Sama_3642)

MetaCyc: 62% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (11/14 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A1SBT8 at UniProt or InterPro

Protein Sequence (454 amino acids)

>Sama_3642 UDP-N-acetylglucosamine diphosphorylase (RefSeq) (Shewanella amazonensis SB2B)
MSLNVVILAAGKGTRMRSDLPKVLHSVAHKPMVQHVIDTARELNADNINLVYGYGGELLK
AKLGEQPLNFVLQAEQLGTGHAVAQAIDHINDEDTVLVLYGDVPLTRKETLEALLAARQP
DGVAVLTVHLDNPTGYGRMVREGGKVVGIVEQKDASPEQLKINEINSGIMALPGKRLKAW
LGRLENNNAQGEFYLTDVIAMAHADGVAIDTAHPANPIETEGANNRVQLAALERAYQARR
AEELMLAGANLRDPARIDIRGDVTVGMDVMIDVNVIFEGTVKLGNNVTIGAGAILIDCDI
ADNADIKPYSIIEGAKLGDSASAGPFARLRPGAELHKDAHIGNFVEMKKAVLGEGSKAGH
LAYLGDAEIGKGVNIGAGTITCNYDGANKHLTVIEDNVFVGSDTQLVAPVVIRKGATLGA
GSTVTHEVGENELVITRVKQRHIQGWQRPVKKPK