Protein Info for Sama_3553 in Shewanella amazonensis SB2B

Annotation: multidrug resistance protein AcrA/AcrE family (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 39 to 372 (334 residues), 299.1 bits, see alignment E=1.6e-93 PF00529: CusB_dom_1" amino acids 48 to 358 (311 residues), 41.5 bits, see alignment E=2.1e-14 PF13533: Biotin_lipoyl_2" amino acids 65 to 110 (46 residues), 28.6 bits, see alignment 2e-10 PF16576: HlyD_D23" amino acids 108 to 290 (183 residues), 43.8 bits, see alignment E=3.8e-15 PF13437: HlyD_3" amino acids 174 to 287 (114 residues), 33.5 bits, see alignment E=1.2e-11

Best Hits

Swiss-Prot: 53% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 100% identity to saz:Sama_3553)

MetaCyc: 53% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SBJ9 at UniProt or InterPro

Protein Sequence (378 amino acids)

>Sama_3553 multidrug resistance protein AcrA/AcrE family (RefSeq) (Shewanella amazonensis SB2B)
MRQMLKIASAIGMVLWVSACQEDAQQAANQQQMPPMEVGTVQVTAAPQNIMVELPGRSRA
FLEAEVRPQVSGIIMERNFVEGREVKEGQSLYKIDDATYKATLESAKADLASAQASLASA
KARAARYEKLVKTNAISKQDFDEADAAYKEALARVKVAEAQLHTANINLNYTEVQAPISG
RIGKSSVTPGALVTANQGQVLATIQQLDPINVDITQSSAQLLALKAKLRAGKLQAADNAE
VQLVLEDGTTYEHKGKLQFAEVSVDENTGSVILRAEFPNPDNVLLPGMYVRAMLNTGVDP
NAILVPQKAITRNTRGEAVAMVVNAESKVEARTVTTAEVINHQWRITSGLNAGDKLIVEG
LQKIRPGAPVTATAAQAK