Protein Info for Sama_3196 in Shewanella amazonensis SB2B

Annotation: acetyl-CoA carboxylase, biotin carboxylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 7 to 115 (109 residues), 144.2 bits, E=6.4e-46
• PF02786: CPSase_L_D2
  • amino acids 120 to 326 (207 residues), 227.3 bits, E=4.5e-71
• PF07478: Dala_Dala_lig_C
  • amino acids 145 to 295 (151 residues), 32.9 bits, E=1.4e-11
• PF02222: ATP-grasp
  • amino acids 145 to 296 (152 residues), 33.5 bits, E=1e-11
• PF02785: Biotin_carb_C
  • amino acids 337 to 442 (106 residues), 103.6 bits, E=1.9e-33

Best Hits

Swiss-Prot: 57% identical to ACCC_ECOLI: Biotin carboxylase (accC) from Escherichia coli (strain K12)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to saz:Sama_3196)

MetaCyc: 57% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (3/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate cycle (3/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (6/18 steps found)
• glyoxylate assimilation (2/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (3/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A1SAJ2 at UniProt or InterPro

Protein Sequence (454 amino acids)

>Sama_3196 acetyl-CoA carboxylase, biotin carboxylase (RefSeq) (Shewanella amazonensis SB2B)
MPTEARFKRVLVANRGEIAVRIIRACHQLGLETVAIYSSADKGALHTLLATHCLCIGPAA
AKDSYLNINAILEAAKLTAADAIHPGYGFLAERAGFARAVTEAGLVFLGPDADTIATMGD
KVSAIKSIKAVGIPTLPGSDGALNDDMNAVEALATDIGYPVLIKASAGGGGRGMRRVDSA
DELASAIALTRSEALAAFGDNTVYLEKFLTHPRHIEFQMLADGEAALCLGERDCSAQRRH
QKLIEEAPALGIAREKIREMADICEAACRRLGYRGVGTFEFLYQDGAFFFIEMNTRIQVE
HTVTEMVTGLDLIAWQLKVALGHPLPPRPQVQGHAIECRINAEAPQSQLPSPGVITQLRV
PGGPGVRWDSYLFPGAMVPAFYDSLIGKLICHGVTREEATARLRQALTELEIQGIAINKS
LHLQLIACDAFQPWQRDIHFVDALSAPGVGQAHL