Protein Info for Sama_3189 in Shewanella amazonensis SB2B

Annotation: secretion protein HlyD (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 signal peptide" amino acids 1 to 48 (48 residues), see Phobius details PF16576: HlyD_D23" amino acids 100 to 316 (217 residues), 96.6 bits, see alignment E=1.4e-31 PF13437: HlyD_3" amino acids 216 to 306 (91 residues), 44 bits, see alignment E=3.3e-15

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_3189)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SAI5 at UniProt or InterPro

Protein Sequence (401 amino acids)

>Sama_3189 secretion protein HlyD (RefSeq) (Shewanella amazonensis SB2B)
MKSIFFSQSIISGANAMSAMNTKASLSALAFLVALAFALPQSKPVWASEGHGHGAAESAA
ETADAHDEDKALRLSQTQRELAGIRVETLSSSGFDLSAVATASLVVDRDRTITLAPQLEV
RVLERHVVPGEEVKQGEPLLTLGGAAIAQAQADYINAATEWSRISRMGESAVSQSSRLQA
RVDYELKRAILESIKMTPKQIKALEAAPDSIGSFKLLAPINGRVQQDVASRGQVLAAGSP
LMQLTDESYLWVEAELTPAQSRGLAMGSYARVSVSGRWLKAKVIGRSHELNPITRTEQVL
LSMANPGHEVHAGEFAELLLPQNSRQGIVLPDAALSRSGDGDWQVFVEDADGFEALEVEI
EARARGLNLVKGLEPGQRVVMSGAFFLASELAKSGFDIHNH