Protein Info for Sama_2877 in Shewanella amazonensis SB2B

Name: lysS
Annotation: lysyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 TIGR00499: lysine--tRNA ligase" amino acids 9 to 499 (491 residues), 662.4 bits, see alignment E=2.1e-203 PF01336: tRNA_anti-codon" amino acids 65 to 135 (71 residues), 42 bits, see alignment E=1.1e-14 PF00152: tRNA-synt_2" amino acids 151 to 496 (346 residues), 315.4 bits, see alignment E=6.2e-98

Best Hits

Swiss-Prot: 100% identical to SYK_SHEAM: Lysine--tRNA ligase (lysS) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 100% identity to saz:Sama_2877)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S9M3 at UniProt or InterPro

Protein Sequence (500 amino acids)

>Sama_2877 lysyl-tRNA synthetase (RefSeq) (Shewanella amazonensis SB2B)
MTEHIQDENKLIAERRAKLEHVRKNCPANGHPNTFRRKHKAAELQAQFGDKTKEELEALG
FQTAIAGRVMAKRGPFLVLQDVSGRIQAYADKGVQADLKDRYAGLDIGDIIGVEGTLHLS
GKGDLYVNMERYELLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEDSREAFKMRSKVV
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFVTHHNALDIEMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLIDLTEEMLSSIAKELLGDTKL
PYGEHVIDFGGPYTRMSMLEAIKHYNPDNATIQAMTYEEVKNIDFMRKLAKDVGIETETF
WSCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDGNDFITDRFEFFIGGREVAN
GFSELNDAEDQDNRFKAQVEAKDAGDDEAMFYDADFITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA