Protein Info for Sama_2827 in Shewanella amazonensis SB2B

Name: obgE
Annotation: GTPase ObgE (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02729: Obg family GTPase CgtA
  • amino acids 3 to 332 (330 residues), 473.1 bits, E=2e-146
• PF01018: GTP1_OBG
  • amino acids 4 to 156 (153 residues), 200.8 bits, E=1.4e-63
• PF01926: MMR_HSR1
  • amino acids 161 to 284 (124 residues), 87.1 bits, E=1.4e-28
• PF02421: FeoB_N
  • amino acids 162 to 327 (166 residues), 29.4 bits, E=8.1e-11

Best Hits

Swiss-Prot: 100% identical to OBG_SHEAM: GTPase Obg (obg) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K03979, GTP-binding protein (inferred from 100% identity to saz:Sama_2827)

MetaCyc: 68% identical to GTPase ObgE (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Obg"

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A1S9H3 at UniProt or InterPro

Protein Sequence (389 amino acids)

>Sama_2827 GTPase ObgE (RefSeq) (Shewanella amazonensis SB2B)
MKFVDEAVIKVQAGDGGSGCVSFRREKYIPDGGPDGGDGGDGGSVYLVADASLNTLIDYR
FERFYLAERGENGRGRDCTGKGGSDLTLRVPVGTRAVDIDTDEVLGDLTEVGQKLLVAKG
GFHGLGNTRFKSSVNRAPRQKTLGTKGEVRELRLELLLLADVGLLGMPNAGKSTFIRAVS
RATPKVADYPFTTLVPNLGVVNPRPGQSFVIADIPGLIEGAAEGAGLGIRFLKHLERCRV
LLHILDIEPIDGSSPAESARAIVAELEKYSPELAAKPRWLVFNKTDLLLEEELQERVDAI
VAELGWEGDVYTMSAATREGTKELAEKLFDFIKSLPDEAAAADPDKEVEFKWDNYHKAQL
DDLNPDFDDSDDDDDFDDDDYDVEVIYQR