Protein Info for Sama_2807 in Shewanella amazonensis SB2B

Name: purT
Annotation: phosphoribosylglycinamide formyltransferase 2 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01142: phosphoribosylglycinamide formyltransferase 2
  • amino acids 12 to 389 (378 residues), 615.5 bits, E=1.6e-189
• PF02222: ATP-grasp
  • amino acids 121 to 292 (172 residues), 178 bits, E=2e-56
• PF02655: ATP-grasp_3
  • amino acids 143 to 283 (141 residues), 25.5 bits, E=2e-09
• PF21244: PurT_C
  • amino acids 319 to 386 (68 residues), 97 bits, E=5.5e-32

Best Hits

Swiss-Prot: 100% identical to PURT_SHEAM: Formate-dependent phosphoribosylglycinamide formyltransferase (purT) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K08289, phosphoribosylglycinamide formyltransferase 2 [EC: 2.1.2.2] (inferred from 100% identity to saz:Sama_2807)

MetaCyc: 69% identical to phosphoribosylglycinamide formyltransferase 2 (Escherichia coli K-12 substr. MG1655)
GARTRANSFORMYL2-RXN [EC: 6.3.1.21]; Acetate kinase. [EC: 6.3.1.21, 2.7.2.1, 2.7.2.15]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• mixed acid fermentation (15/16 steps found)
• Bifidobacterium shunt (13/15 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-threonine degradation I (6/6 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• ethanolamine utilization (4/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
• pyruvate fermentation to acetate I (2/3 steps found)
• pyruvate fermentation to acetate VII (2/3 steps found)
• glycine degradation (reductive Stickland reaction) (1/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• (S)-propane-1,2-diol degradation (3/5 steps found)
• pyruvate fermentation to acetate and lactate II (2/4 steps found)
• acetyl-CoA fermentation to butanoate (4/7 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• methanogenesis from acetate (2/6 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• gallate degradation III (anaerobic) (3/11 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (11/24 steps found)
• superpathway of methanogenesis (2/21 steps found)
• superpathway of L-lysine degradation (8/43 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• One carbon pool by folate
• Propanoate metabolism
• Purine metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2, 2.7.2.1

Use Curated BLAST to search for 2.1.2.- or 2.1.2.2 or 2.7.2.1 or 2.7.2.15 or 6.3.1.21

Sequence Analysis Tools

See A1S9F3 at UniProt or InterPro

Protein Sequence (392 amino acids)

>Sama_2807 phosphoribosylglycinamide formyltransferase 2 (RefSeq) (Shewanella amazonensis SB2B)
MIGTPYTQGATRAMLLGSGELGKEVAIELQRLGVEVIAVDRYPNAPAMQVAHRSHVINML
DGEALKALIASEKPHLVIPEIEAIATQTLVELEAEGLKVVPTARATRLTMDREGIRRLAA
ETLALPTSPYVFCDSLTELQAALKVTGIPCVVKPVMSSSGKGQSVIRSPGDVQKAWDYAQ
SGGRAGEGRIIVEGFIDFDYEITLLTISAVDGIHFCAPIGHRQEDGDYRESWQPQAMSSL
ALDRAQHIAREVVQNLGGFGLFGVELFIKGDEVYFSEVSPRPHDTGMVTLISQDLSEFAL
HVRAILGLPVGTIVQRGPSASAVILVEGESSNIGYSGLAEALAQPNTQLRLFAKPEIHGR
RRLGVALARGTSIDEALQTALASAGCIKVHFN