Protein Info for Sama_2185 in Shewanella amazonensis SB2B

Annotation: cold-active serine alkaline protease (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 803 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF05922: Inhibitor_I9" amino acids 44 to 129 (86 residues), 29.5 bits, see alignment E=2.4e-10 PF00082: Peptidase_S8" amino acids 155 to 345 (191 residues), 99.6 bits, see alignment E=5.4e-32 amino acids 480 to 546 (67 residues), 55.1 bits, see alignment E=1.9e-18 PF02225: PA" amino acids 382 to 473 (92 residues), 27.3 bits, see alignment E=7.8e-10 PF04151: PPC" amino acids 597 to 663 (67 residues), 73.6 bits, see alignment E=4.9e-24 PF01483: P_proprotein" amino acids 723 to 802 (80 residues), 83.1 bits, see alignment E=3.1e-27

Best Hits

KEGG orthology group: K14645, serine protease [EC: 3.4.21.-] (inferred from 100% identity to saz:Sama_2185)

Predicted SEED Role

"Alkaline serine protease"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S7N4 at UniProt or InterPro

Protein Sequence (803 amino acids)

>Sama_2185 cold-active serine alkaline protease (RefSeq) (Shewanella amazonensis SB2B)
MTMKHKKTLALGLSTLALAVSAGVHAAPTDAVFAKGENSPLPKRYVVKFKNNAATELYNA
EDTLNSMQYQPRSHEVYGHHRALNAASAKEMKRIGRSNAYTVKLDNNGIKALRARADVEF
VEEDVPRRLLAETTPWGQTFVGATQLADNLSGNRTICIIDSGYDRGHADLSGNNVTGTNN
SGTGNWFEPGNNNAHGTHVAGTIAAIANGEGVVGVLPNQNANIHIIKVFNEAGWGYSSSL
VSAVDTCVANGANVVSMSLGGASSSTTERNALAAHYNNGVLLIAAAGNDGDNTHSYPASY
DSVVSVAAVDSTKQHAAFSQYTNQVEVSGPGEAILSTVTRGEGRLSDIVVAGQSYFNNGV
VPHNRLVKSGTSYAPAPINGTVTATLAECSVSGSTFNCGNMAGKVCLVERVGNQGSSYPE
INAAKACQNAGASATIVYSNTALPGLQNPFVVDTNSELTKVSVSVDRATGLALRSHIGSS
VTVSSEGGKDYEYYNGTSMATPHVSGVATLVWSYHPECSAAQVRAALRTTAEDLDVAGRD
DKTGYGLVDAVAAKEYLDASCDGPTNGGGNNGGSDAEMTNGVAKSNLAGAKNEELHFFID
VPAGATNLNFAMSGGSGDADLYVQYGAAPTTSSYDCRPWKGGNTESCPITTAQAGTYYVM
VQGYSAFSGVNLVASYTAPTGGSTGGTTGPATYTNTDNYSIPDNNTAGISSPIDVSRTGD
AGTVTVKVNIVHTYIGDLQVELITPSGQVAVLHDNTGAGTDNINKTYTVNATGVESQGTW
KLKAVDSSRRDTGYIDSWEISFQ