Protein Info for Sama_2160 in Shewanella amazonensis SB2B

Annotation: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 13 to 296 (284 residues), 446.9 bits, see alignment E=2.9e-138 TIGR00536: methyltransferase, HemK family" amino acids 15 to 299 (285 residues), 301.4 bits, see alignment E=5.3e-94 PF06325: PrmA" amino acids 131 to 209 (79 residues), 30.3 bits, see alignment E=9.4e-11 PF05175: MTS" amino acids 132 to 217 (86 residues), 53.9 bits, see alignment E=5.4e-18 PF13847: Methyltransf_31" amino acids 135 to 275 (141 residues), 38.7 bits, see alignment E=2.6e-13 PF09445: Methyltransf_15" amino acids 135 to 211 (77 residues), 27 bits, see alignment E=9.6e-10 PF13649: Methyltransf_25" amino acids 137 to 220 (84 residues), 37.6 bits, see alignment E=8.8e-13

Best Hits

Swiss-Prot: 88% identical to PRMB_SHEON: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 100% identity to saz:Sama_2160)

MetaCyc: 66% identical to ribosomal protein L3 N5-glutamine methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-1241 [EC: 2.1.1.298]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.298 or 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S7K9 at UniProt or InterPro

Protein Sequence (314 amino acids)

>Sama_2160 N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (RefSeq) (Shewanella amazonensis SB2B)
MDKIFVDEAVTELRTIGDMLRWAVSRFNDAKVYYGHGTDNAWDEAVALVFHALHLPDDLG
REVIGANLTSSEKHKIVELIIRRVRERLPVPYLTNRAWFAGLEFYVDERVLVPRSPIAEM
IEDRFGPWLYNKPVNRVLDLCTGSGCIAIACAHTFEDAEVDALDISEDALDVAQINVESH
GLVERVFPMQSDLFSAIPKGPQYDLIVSNPPYVDEEDIGDMPDEYHHEPAIGLASGRDGL
DITKRILANAADYLTPSGILVVEVGNSMVHLMEQFPEVPFTWVSFERGGDGVFVLTYDQL
VENESLFAIYKDRE