Protein Info for Sama_2149 in Shewanella amazonensis SB2B

Annotation: FolC bifunctional protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 34 to 420 (387 residues), 339 bits, E=2.6e-105
• PF08245: Mur_ligase_M
  • amino acids 57 to 199 (143 residues), 35.2 bits, E=1.3e-12
• PF02875: Mur_ligase_C
  • amino acids 293 to 411 (119 residues), 74.3 bits, E=1.7e-24

Best Hits

Swiss-Prot: 53% identical to FOLC_ECOLI: Dihydrofolate synthase/folylpolyglutamate synthase (folC) from Escherichia coli (strain K12)

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 100% identity to saz:Sama_2149)

MetaCyc: 53% identical to bifunctional folylpolyglutamate synthetase / dihydrofolate synthetase (Escherichia coli K-12 substr. MG1655)
Tetrahydrofolate synthase. [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; Dihydrofolate synthase. [EC: 6.3.2.17, 6.3.2.12]

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of chorismate metabolism (50/59 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See A1S7J8 at UniProt or InterPro

Protein Sequence (421 amino acids)

>Sama_2149 FolC bifunctional protein (RefSeq) (Shewanella amazonensis SB2B)
MTQEQAMSPGLGATLGEWLDYLLAIHPTEIDMGLARVTEVAQRLRVKSLGDILVVTVGGT
NGKGSTCAMLENILRRAGYRVGVYSSPHILHYNERIRIDGVDALDADIINAFERIEEARG
DISLTFFEFATLTGLLLFKQAGLDVAILEVGLGGRLDATNLVDADIAVVTSVDLDHQAYL
GNTRESVGREKAGIFRAGRPAIVGEPDMPSTVAEVANHCGALLYRVGQDFRYQREQSSWS
YQGKLRSLTNMPLPQLPLPNAATVIATCEAIGERLNLSDTVVIEGIVTARLAGRLEELSQ
SPLVIADVAHNPHAARFLKSELVRWPGKRVLALCGMLKDKDYREVLNIMHPAVADWYLVT
LEGERGTRASELAAALPAGAKAHTFDTIEQAREAILKDAGEGDVVIVFGSFYTVSGFKSL
A