Protein Info for Sama_2072 in Shewanella amazonensis SB2B

Annotation: phosphoenolpyruvate synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 5 to 787 (783 residues), 1246.2 bits, E=0
• PF01326: PPDK_N
  • amino acids 17 to 342 (326 residues), 422.1 bits, E=2.6e-130
• PF00391: PEP-utilizers
  • amino acids 382 to 454 (73 residues), 97.3 bits, E=5e-32
• PF02896: PEP-utilizers_C
  • amino acids 481 to 784 (304 residues), 211.6 bits, E=2.3e-66

Best Hits

Swiss-Prot: 77% identical to PPSA_ECOLI: Phosphoenolpyruvate synthase (ppsA) from Escherichia coli (strain K12)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 80% identity to alt:ambt_09865)

MetaCyc: 77% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• gluconeogenesis I (13/13 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See A1S7C1 at UniProt or InterPro

Protein Sequence (789 amino acids)

>Sama_2072 phosphoenolpyruvate synthase (RefSeq) (Shewanella amazonensis SB2B)
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLANAGVQVPGGFATTAHAFNEFLEQSGV
NQKIYDILDTLDVDDVNALAKVGAQIRQWVIETPFQPELEQAIHEAYNKLSGETTEASFA
VRSSATAEDMPDASFAGQQETFLNVKGYDAVLVAIKHVFASLFNDRAISYRVHQGYDHRG
VALSAGVQRMVRSDKAASGVMFTIDTESGNDDVVFVTSSYGLGEMVVQGAVNPDEFYVHK
PTLTAGHQAVVRRNIGSKLIQMVYSDDLSHGKQVKIEDVSVENRRQFSINDAEVMELAKQ
AIIIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGVVISEGR
AIGHKVGSGVAKVLKSIEEMDKIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
IIARELGVPAVVGCGDVTDRIQNGQMVTVSCAEGDTGYIYDGKQEFEVISSRVDSMPSLP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQEAALQEEINE
MIAGYDSPVEYYVARLVEGIATIAAAFHPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEIMIPFVRTLGEAEQVIELLKQ
EGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNEAVKALLAMAIKAAKAKGAYVGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDT
WLYLAEAHG