Protein Info for Sama_1996 in Shewanella amazonensis SB2B

Annotation: diacylglycerol kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 123 transmembrane" amino acids 32 to 52 (21 residues), see Phobius details amino acids 58 to 79 (22 residues), see Phobius details amino acids 98 to 122 (25 residues), see Phobius details PF01219: DAGK_prokar" amino acids 18 to 118 (101 residues), 125.3 bits, see alignment E=3.9e-41

Best Hits

Swiss-Prot: 52% identical to KDGL_SHIFL: Diacylglycerol kinase (dgkA) from Shigella flexneri

KEGG orthology group: K00901, diacylglycerol kinase [EC: 2.7.1.107] (inferred from 100% identity to saz:Sama_1996)

MetaCyc: 52% identical to diacylglycerol kinase (Escherichia coli K-12 substr. MG1655)
Diacylglycerol kinase. [EC: 2.7.1.107]

Predicted SEED Role

"Diacylglycerol kinase (EC 2.7.1.107)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.1.107)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.107

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S745 at UniProt or InterPro

Protein Sequence (123 amino acids)

>Sama_1996 diacylglycerol kinase (RefSeq) (Shewanella amazonensis SB2B)
MKPANNHGFRRVIRATGFSFKGLKSAWVHEAAFRQELILACVMVPFALWLDVSTVEKLLM
IMAVFIVLITELLNSAIEAVVDRIGDEIHPLSGQAKDIASAAVFMSLALCALVWAFILLP
LLW