Protein Info for Sama_1946 in Shewanella amazonensis SB2B
Annotation: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to HIS4_SHEAM: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
KEGG orthology group: K01814, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [EC: 5.3.1.16] (inferred from 100% identity to saz:Sama_1946)MetaCyc: 59% identical to 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (Escherichia coli K-12 substr. MG1655)
isomerase. [EC: 5.3.1.16]
Predicted SEED Role
"Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)" in subsystem Histidine Biosynthesis (EC 5.3.1.16)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
- L-histidine biosynthesis (10/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Histidine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 5.3.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1S6Z5 at UniProt or InterPro
Protein Sequence (245 amino acids)
>Sama_1946 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq) (Shewanella amazonensis SB2B) MIIPAIDLIDGQVVRLFQGDYAKKTEFSLDPKNQLLSYQQDGAALLHLVDLTGAKDPDKR QTKLIEEIAASLSTPLQVGGGIRSAADVDALLNAGVARVVIGSLAVKSPEVVLRLFDKYG GDAICLALDVNIDADGNKMVAVHGWQQGGGKSLESLVDTFMPAGLKHALVTDISRDGTMT GANTALYCELAERFNEVQWQASGGVATLDDVKAVKTSGASGIIIGKALLTGVFSAKEAIA CWPNV