Protein Info for Sama_1828 in Shewanella amazonensis SB2B

Annotation: excinuclease ABC subunit B (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 TIGR00631: excinuclease ABC subunit B" amino acids 6 to 665 (660 residues), 1075.6 bits, see alignment E=0 PF04851: ResIII" amino acids 17 to 93 (77 residues), 31.8 bits, see alignment E=4.7e-11 PF17757: UvrB_inter" amino acids 160 to 250 (91 residues), 116.3 bits, see alignment E=1.9e-37 PF27431: UvrB_3rd" amino acids 255 to 316 (62 residues), 94.1 bits, see alignment E=1.3e-30 PF00271: Helicase_C" amino acids 440 to 546 (107 residues), 70.4 bits, see alignment E=5.3e-23 PF12344: UvrB" amino acids 553 to 594 (42 residues), 75.8 bits, see alignment 6.3e-25 PF02151: UVR" amino acids 636 to 668 (33 residues), 34.1 bits, see alignment (E = 5.9e-12)

Best Hits

Swiss-Prot: 73% identical to UVRB_SALTI: UvrABC system protein B (uvrB) from Salmonella typhi

KEGG orthology group: K03702, excinuclease ABC subunit B (inferred from 100% identity to saz:Sama_1828)

MetaCyc: 72% identical to UvrABC excision nuclease subunit B (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit B" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S6M7 at UniProt or InterPro

Protein Sequence (672 amino acids)

>Sama_1828 excinuclease ABC subunit B (RefSeq) (Shewanella amazonensis SB2B)
MSEQVFQLSSHYSPAGDQPTAIAKLIDGLESGLACQTLLGVTGSGKTFTVANVIATLGRP
TIIMAPNKTLAAQLYGEMKEFFPNNAVEYFVSYYDYYQPEAYVPASNTFIEKDASVNAHI
EQMRLSATKALLERRDVIIVASVSAIYGLGDPDSYMKMLLHLRQGDFMGQRDILRRLSEL
QYSRNDIDLQRGTFRVRGEVIDIFPADSERDAIRVELFDDEIERISEFDPLTGQVTKRLA
RATVYPKTHYVTPREKILEAIDQIKDELRVRKQQFLDNHKLIEAQRITERVQYDVEMMTE
LGYCSGIENYSRYLSGRAEGEGPPTLLDYLPADGLMIIDESHNTVPQIGAMYKGDRSRKT
TLVEYGFRLPSALDNRPLKFEEFESLMPQTIFVSATPGKYELEKSGDDIAEQVVRPTGLI
DPELEVRPVAIQVDDLLSEVAKRVAVNERVLVTTLTKRMAEDLTDYLDEHGVKVRYLHSD
IDTVERMEIIRDLRLGVFDVLVGINLLREGLDMPEVSLVCILDADKEGFLRSERSLIQTI
GRAARNVNGKVILYADRITDSMARAMAETERRRTKQMQFNEEHGIIPRGVVKKITDVMDV
GERSAPSRGAVKASGRSRDVGKPSMRQDPHQIAVTIDALEKQMHQHARDLEFEQAAALRD
EIHGLRQQLLEA