Protein Info for Sama_1600 in Shewanella amazonensis SB2B

Name: rlmL
Annotation: 23S rRNA m(2)G2445 methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 PF22020: RlmL_1st" amino acids 3 to 57 (55 residues), 54.2 bits, see alignment 5.3e-18 PF02926: THUMP" amino acids 67 to 152 (86 residues), 52.7 bits, see alignment E=2.1e-17 PF01170: UPF0020" amino acids 162 to 371 (210 residues), 169.8 bits, see alignment E=2.8e-53 PF10672: Methyltrans_SAM" amino acids 459 to 664 (206 residues), 91.5 bits, see alignment E=2.6e-29 PF03602: Cons_hypoth95" amino acids 541 to 666 (126 residues), 34.2 bits, see alignment E=9.2e-12

Best Hits

Swiss-Prot: 100% identical to RLMKL_SHEAM: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 100% identity to saz:Sama_1600)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S601 at UniProt or InterPro

Protein Sequence (708 amino acids)

>Sama_1600 23S rRNA m(2)G2445 methyltransferase (RefSeq) (Shewanella amazonensis SB2B)
MFNFFAAAPKGFEYALAKELETLGAENVRESVAGVYFSASLAQGYQITLWTRLASRIVLI
LFTGECQSAEQLYNAAYTIDWPSHFSNRSTFSIDFHGTGGFINNSQFGALKIKDAVVDRF
RDDDLSRPDVVKGHPDMRIDAHYGRGKITIGINFSGAALHQRGYRGNTGEAPLKENLAAN
MLYRSGWADNPVTLLDPFCGSGTVLIEAALMACDIAPGLMRERFGFEHWRRHDQALWQQV
IEEAKARASLGKTRCQLKFYGSDIDSRVVALAKRNANSAGVFDFIDFKVANALNLEPPVA
EGMVLTNPPYGERLGNVTSLLQLYFQLGEKFKQSYGGWKLGLLCSDMELVSSLKLKADKQ
MKMYNGALECAFNLYTLHATSTRRDIPVDTTGQGGEIAAPFANRLKKNLKQLEKWAKREG
IDSYRLYDADIPEYNVAVDRYLDYVVVQEYAAPSAIPEAVTKRRLTDVLLALPRALGIDP
DKIVLKTRERQKGTNQYQKLDGEKLELVTTEYGCSFKLNLTDYLDTGLFLDHRLTRKLVG
EKAKNRDVLNLFAYTGSASVHAGKGGAKSVTTVDMSNTYLNWAKDNFMLNGLIGRQYQFE
QADCLQWIRDCERQFDLIFIDPPTFSNSKRMEDSFDVQRDHVDLLASLKKLLRPGGEIVF
SNNKRKFKMDMEALAAAGLKAVNIDDKVLPMDYARNPQIHNCWVVTHG