Protein Info for Sama_1478 in Shewanella amazonensis SB2B

Annotation: arsenite efflux pump ACR3 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 358 transmembrane" amino acids 22 to 43 (22 residues), see Phobius details amino acids 52 to 72 (21 residues), see Phobius details amino acids 89 to 112 (24 residues), see Phobius details amino acids 118 to 137 (20 residues), see Phobius details amino acids 149 to 172 (24 residues), see Phobius details amino acids 192 to 214 (23 residues), see Phobius details amino acids 227 to 249 (23 residues), see Phobius details amino acids 261 to 282 (22 residues), see Phobius details amino acids 294 to 317 (24 residues), see Phobius details amino acids 323 to 345 (23 residues), see Phobius details TIGR00832: arsenical-resistance protein" amino acids 15 to 344 (330 residues), 445.7 bits, see alignment E=5.3e-138 PF01758: SBF" amino acids 60 to 256 (197 residues), 181.8 bits, see alignment E=5.8e-58

Best Hits

Swiss-Prot: 59% identical to ARSB_BACSU: Arsenite resistance protein ArsB (arsB) from Bacillus subtilis (strain 168)

KEGG orthology group: K03325, arsenite transporter, ACR3 family (inferred from 100% identity to saz:Sama_1478)

MetaCyc: 59% identical to arsenite efflux transporter Acr3 (Bacillus subtilis)
TRANS-RXN0-264

Predicted SEED Role

"Arsenical-resistance protein ACR3" in subsystem Arsenic resistance

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S5M9 at UniProt or InterPro

Protein Sequence (358 amino acids)

>Sama_1478 arsenite efflux pump ACR3 (RefSeq) (Shewanella amazonensis SB2B)
MTTNRCIDVPVSQKMSFLDRNLTLWIFVAMALGVALGYLFPGIAQLNESMSVGSTNVPLA
IGLILMMYPPLAKVNYATMGTMARDRRAITLSLLMNWLIGPILMFAIALLFLRDYPGYMV
GLTLIGLARCIAMVLVWNDLAGGSKEYGAGLVALNSAFQILTYSFMAWLFITVLPPLFGL
QGVAVDITMLDIAKSVLIYLGIPFVAGFLSRKYLIRAKGEQWYTERFIPAISPITLIALL
ATIVLMFSLKGEMIVQLPMDVLRVALPLVLYFVLMFFSSFYIGKKLGLPYDKNASIAFTA
TGNNFELAIAVSIAVFGLNSDQAFAGVIGPLVEVPVLILLVNVALRFKQGYTRSLSAS