Protein Info for Sama_1421 in Shewanella amazonensis SB2B

Annotation: HlyD family secretion protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 38 to 343 (306 residues), 223.4 bits, see alignment E=1.8e-70 PF16576: HlyD_D23" amino acids 64 to 262 (199 residues), 85.3 bits, see alignment E=7.7e-28 PF13533: Biotin_lipoyl_2" amino acids 68 to 109 (42 residues), 43.2 bits, see alignment 5.2e-15 PF13437: HlyD_3" amino acids 160 to 256 (97 residues), 64.4 bits, see alignment E=2.9e-21

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_1421)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S5H2 at UniProt or InterPro

Protein Sequence (354 amino acids)

>Sama_1421 HlyD family secretion protein (RefSeq) (Shewanella amazonensis SB2B)
MEARKSFKLALPLLLVGVLAACGQEEAKKEEEKYAIPVETQLVVQGNVSSFYSTTATLEA
PVEAHVVTRIAGLIEALHVEEGDRVKQGQLLAVIDAKRQQYELDRSEAEVLIIEQELARV
KKMKSKDYVSADSIAKLEYNLQAAKARLDLAKLQVKESQIVSPVDGIIAKRFVKAGNMAK
EFEELFYVVNQDELHGIVHLPEQQLSSLRLGQHADIYASGQKNQAVNAEVLRISPVVDAQ
SGTFKVTLKVPNQNGILKSGMFTRVELKYDTHENVITVPYNAIINQDNKQTLYVVQDTTA
ERREVELGYREGNMVEVVAGIAPGEHLVIRGQQNLKDQSLVEIITPLDVASVKK