Protein Info for Sama_1396 in Shewanella amazonensis SB2B

Annotation: glucan endo-1,3-beta-D-glucosidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 884 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00722: Glyco_hydro_16" amino acids 88 to 277 (190 residues), 85.7 bits, see alignment E=2.8e-28

Best Hits

KEGG orthology group: K01199, glucan endo-1,3-beta-D-glucosidase [EC: 3.2.1.39] (inferred from 100% identity to saz:Sama_1396)

Predicted SEED Role

"Beta-glucanase precursor (EC 3.2.1.73)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.73)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.39 or 3.2.1.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S5E7 at UniProt or InterPro

Protein Sequence (884 amino acids)

>Sama_1396 glucan endo-1,3-beta-D-glucosidase (RefSeq) (Shewanella amazonensis SB2B)
MMMKNSGTSKYLSLGLLAAALQGCGGDTNTSTDLGSVNLEGPATGWELVWSDEFDGSSID
AAKWAHDVDCAGGGNQESQCYTADEANSFVADGMLNIVALKAPEGAEKPYTSARLTTKGK
ADFTYGRFEMRAKLPSGQGSWPAFWMLPTDNVYGIWPRSGEIDIVEAVNLKAATVEGTPE
AYVHGTLHYGREWPKNEQSGKAYLLPNGVNPADDFHTYAIEWQQGEIRWYVDDYLYATQR
RSELRYNSKGQAVGLAHKGWFTEYYDQSSGDLQLSWTDAPFDEKFHLIVNFAVGGNWPAS
VNETGIDDTAFNENNRYQIDFVRVYECAANPDTGKGCETVRPGYDNPDDALVEGKAPVPV
PPSDGTPKDLLIFDSTPNPNWAAWDCCGGSTPSLVEDADKGAVYRFVVGDSPTVNGFISR
AAFITDPAGVPSPFDAAPIEASGVLSFAMKVVSAPSNADSTWMMKVESNEGATAVELPLT
ASSEGVAPAVGQWQTFSFPLSELAAKGLDLSAIDVVMVFPAWGTGSGAEYLLDEVKIARP
DTSLPSLTIFTDNENPDWPMWDCCGGSTPQVVSDDAEHGIVSEFSIGASPTVMGYINRTE
LGGGGNPFDASSLYDNGVVQFDLKVVTAPNTVDAPWLFKVESNNAASAVELPLTSSVEGM
APSAQWQTFSFRLSTLADAGLDLSAIDVLMVFPAWGQGEGAVYRIDNVKIHQPGSGGSMN
ALSLFADTVADAWSIWDCCGGSTPTVEADDAAHGAVAEYVIGAQPTVVGFFADDGVYYDA
SAAVETGVVRFEMKLVSAPNDPGSVWKFKIESGDASTAVELNLTDSVEGSAPVVGQWQTY
TFPLKTLTDMGLDASAIDVVMVFPAWGTGEGAVFRLDNALIGTP