Protein Info for Sama_1275 in Shewanella amazonensis SB2B

Annotation: anaerobic ribonucleoside triphosphate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 3 to 88 (86 residues), 67.5 bits, E=1.9e-22
• PF13597: NRDD
  • amino acids 115 to 704 (590 residues), 645.8 bits, E=1e-197
• TIGR02487: anaerobic ribonucleoside-triphosphate reductase
  • amino acids 115 to 704 (590 residues), 653.5 bits, E=1.5e-200
• PF01228: Gly_radical
  • amino acids 580 to 685 (106 residues), 104.7 bits, E=5.7e-34

Best Hits

Swiss-Prot: 78% identical to NRDD_SALTY: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 79% identity to ddc:Dd586_0360)

MetaCyc: 78% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Sequence Analysis Tools

See A1S526 at UniProt or InterPro

Protein Sequence (705 amino acids)

>Sama_1275 anaerobic ribonucleoside triphosphate reductase (RefSeq) (Shewanella amazonensis SB2B)
MPVVIKRDGCRAAFDETRLRDAVIAAQGAAGVEDKDYAATVASVVAAEVAQRDEVAIHEL
QDAVENCLMSGPYKEVARHYIEYRHDRDVCREATSRLNLEIRSLVEQTNAALLHENANKD
SKVIPTQRDLLAGIVARHYAKRHILPKAVVAAHEAGEIHYHDLDYSPFFPMFNCMLIDLA
GMLTKGFKMGNAEIETPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLSPYVAKSYD
KHYQTALHWGITDAVAYARALTEKECHDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTSW
ESRLIQQSILKVRMAGLGKNRKTAVFPKLVFAIRDGINHKQGDCNYDIKRLALKCATERM
YPDILNYEQVVRVTGSFKTPMGCRSFLGVYEKDGELEHEGRNNLGVVSLNLPRIAIEAGG
DESRFYSLLEERLQVARQALDSRIERLKGVKARVAPILYMEGACGVRLRPDDDIEPIFKN
GRASISLGYIGLHETVNALYGTQEHVYDSEELRQKAVAIVARLKQATEQWKQETGYGFSL
YSTPSESLCSRFAKLDIKAFGLIQGVTDKGYYTNSFHLDVEKKVSPFDKIDFEMPYPELA
SGGFICYGEYPNMQHNIDALEDVWDYSYTRVPYYGTNTPIDECYDCGFTGEFDCTSKGFV
CPVCGNHEPSRVSVTRRVCGYLGSPDARPFNHGKQEEVKRRVKHL