Protein Info for Sama_1048 in Shewanella amazonensis SB2B

Name: alaS
Annotation: alanyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 68% identical to SYA_ECODH: Alanine--tRNA ligase (alaS) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 68% identity to esc:Entcl_1030)

MetaCyc: 68% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

superpathway of chorismate metabolism (50/59 steps found)
tRNA charging (21/21 steps found)
shinorine biosynthesis (3/8 steps found)
enterobactin biosynthesis (5/11 steps found)
vanchrobactin biosynthesis (2/8 steps found)
bacillibactin biosynthesis (4/12 steps found)
ergotamine biosynthesis (4/12 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
superpathway of ergotamine biosynthesis (5/18 steps found)
sulfazecin biosynthesis (1/16 steps found)
phosalacine biosynthesis (2/25 steps found)
phosphinothricin tripeptide biosynthesis (2/25 steps found)
corallopyronin A biosynthesis (2/30 steps found)
colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S4E9 at UniProt or InterPro

Protein Sequence (900 amino acids)

>Sama_1048 alanyl-tRNA synthetase (RefSeq) (Shewanella amazonensis SB2B)
MSPKFLSAARGRGCGLSYQAYPTQDVFMYQTTAALRSAFLEYFRQQGHQVVDSSSLVPGN
DPTLLFTNAGMNQFKDCFLGMEKRSYVRAATAQRCVRAGGKHNDLDNVGYTARHHTFFEM
LGNFSFGDYFKEDAIRFAWEFLTGTLKLPKEKLCVTVYHTDDEAFDIWNKQIGVPAENII
RIGDNKGAPFASDNFWQMGDTGPCGPCTEIFYDHGEHIWGGRPGSPEEDGDRFIEIWNIV
FMQYNRQSDGTLDPLPKPAVDTGMGIERISAIMQGVHSNYEIDIFQKLIAKAAEIIGVSD
LENKSLRVIADHIRSCAFLVADGVMPSNEGRGYVLRRIIRRAVRHGNKLGATDTFFYKLV
PTLIEAMGDAAKELKATQTIVEKALKAEEEQFARTLERGLGMLDAALADLKGDTLDGETV
FKLYDTYGFPVDLTADVCRERNLKVDEEGFNAAMAEQRSRAQAAGQFSADYNAALKIDTA
TDFCGYTELAAESKVVALYRDGESVDAAEAGAVLVVVLDNTPFYAESGGQVGDKGQLLAE
GVEFNVADTQKFGQGIGHKGQLALGSIKVGDSLKAMVDKKLRHRTELNHSVTHLLHAALR
QVLGTHVTQKGSLVDPERLRFDFSHFEGVKREELKAVEDLVNTQIRRNHELNFAEMDIEE
AKAKGAMALFGEKYDSKVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEGGIAAGIRRI
EAVTGAAAMAYVAQQQAELEEAAALLKGDVASVVAKLKAQLDKARQLEKENAQLKDKLAA
AASADLAGDAQEINGVKVLVKRLEGVEAGALRGLQDELKQKLKSGVVLLAIGSGDKVNLI
AGVTQDLTGKVKAGELVAMVAAQVGGKGGGRPDMAQAGGSEPAKLDGALASVVPWLNERL