Protein Info for Sama_1031 in Shewanella amazonensis SB2B

Annotation: hybrid sensory histidine kinase BarA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 941 transmembrane" amino acids 14 to 36 (23 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details PF09984: sCache_4" amino acids 37 to 195 (159 residues), 171.8 bits, see alignment E=2.4e-54 PF00672: HAMP" amino acids 217 to 267 (51 residues), 31.5 bits, see alignment 5.4e-11 PF00512: HisKA" amino acids 312 to 375 (64 residues), 79 bits, see alignment 6.8e-26 PF02518: HATPase_c" amino acids 423 to 539 (117 residues), 99.3 bits, see alignment E=5.7e-32 PF00072: Response_reg" amino acids 695 to 805 (111 residues), 89 bits, see alignment E=7.2e-29 PF01627: Hpt" amino acids 841 to 924 (84 residues), 48.9 bits, see alignment E=1.9e-16

Best Hits

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 100% identity to saz:Sama_1031)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S4D2 at UniProt or InterPro

Protein Sequence (941 amino acids)

>Sama_1031 hybrid sensory histidine kinase BarA (RefSeq) (Shewanella amazonensis SB2B)
MNTAHNMTKYSLRSWVLVLALAPTILVGILLGSYFTINRFYDLEDTLKDMGSNIIEPLAI
SAEQSLSTNNREITKILLARAQLNKSTLVKSIAIFDINNQLFVTSHYHKDFEVMRYKEAL
ESLKETQFEHEGDTLIVRTPIFAISSKQPMGDNIDEGSGRFSEAVVMPDNSNAALLGYVS
MMLNKEKALLEQHRAAVAAFIIVLIGVQLNLLFTFRLVKNVTQPITDMVKLVGKIREGKL
DARLEGNLIGELDLLNRGINAMAASLSEYHDEMQQNIDQATSDLRETLEQIEIQNIALDR
AKKDAQEASRIKSEFLANMSHELRTPLNGVIGFARQLLKTPLHSSQMEYIKTIERSATSL
LQIINDILDFSKLEANKMVLETMPFGLRASLEDTINLLAGSAHAKGLEFVVDVDANVPEN
LSGDVMRLGQVMTNLLGNAIKFTEEGSVHLKISLASLEDNNLTLRCEVTDTGIGIDPEQQ
ELLFQAFGQADSSISRRYGGTGLGLVITKRLVVQMMGGRIGFHSEVGKGSSFWFTVPLQL
SPFPVHDTLPLEKLIGHSVLLFEPRKLTRESIARKLKDWHMMVTLASNEDELERHLDDRH
YDAILLSGQSCESCEDLQRHLSRVKDHTGSLLLLNDCLDPEVYTQEMEPFVDLVLQLPVS
ELKLAQNLIFPPVKKNAAPKPALATKPSARHPLNVLAVDDNLANLKLIDTLLKELVANVV
AVSSGDQAVALAKSRSFDLIFMDIQMPGTDGVTATRLIRQDSLNRNTPIIAVTAHAINEE
RERIQASGMDGYLPKPIDEAALKGVISRWLTRPKFTHFDSNTLNWELCLSQANQKEDLAI
DMLKMMLESLPGTVNDIELAMARSDNESMLYTVHKLHGASCYCGVPTTQKLCQQIESALK
GGADVADVEPEILELLDELTKVESAATQVISQMSAEVPNEF