Protein Info for Sama_0922 in Shewanella amazonensis SB2B

Annotation: integral membrane protein MviN (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 521 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 33 to 41 (9 residues), see Phobius details amino acids 52 to 70 (19 residues), see Phobius details amino acids 92 to 116 (25 residues), see Phobius details amino acids 135 to 159 (25 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 198 to 218 (21 residues), see Phobius details amino acids 246 to 274 (29 residues), see Phobius details amino acids 282 to 301 (20 residues), see Phobius details amino acids 322 to 346 (25 residues), see Phobius details amino acids 359 to 380 (22 residues), see Phobius details amino acids 392 to 411 (20 residues), see Phobius details amino acids 416 to 437 (22 residues), see Phobius details amino acids 448 to 472 (25 residues), see Phobius details amino acids 484 to 510 (27 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 4 to 512 (509 residues), 455 bits, see alignment E=1.7e-140 PF03023: MurJ" amino acids 30 to 476 (447 residues), 479.8 bits, see alignment E=7.5e-148 PF13440: Polysacc_synt_3" amino acids 202 to 346 (145 residues), 29 bits, see alignment E=6.6e-11

Best Hits

Swiss-Prot: 71% identical to MURJ_VIBCH: Probable lipid II flippase MurJ (murJ) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03980, virulence factor (inferred from 100% identity to saz:Sama_0922)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S423 at UniProt or InterPro

Protein Sequence (521 amino acids)

>Sama_0922 integral membrane protein MviN (RefSeq) (Shewanella amazonensis SB2B)
MSKKLLKSGLVVSAMTFISRVLGLVRDMVVANLMGAGASADVFFFANKIPNFLRRLFAEG
AFAQAFVPVLTEYQEKRSSEETRELISKVAGTLGGLVTIVTLLGVIGSPILAALFGGGWF
LDWLEGGENGAKFELAALMLKITFPYLWFITFTALAGSILNTRGRFAVSAFTPVFLNIAI
IAFALWLSPQLEQPEIGLAWGVFAGGLIQFLFQIPFLYRERALVKPSWGWHHPGVVKIRT
LMIPALFGVSVSQINLLFDTFIASFLMTGSISWLYYSDRLLEFPLGLFGIAIATVVLPAL
SKKHVNDEGDGFAKTMDWGVRAILLLGMPAMVGLIVLAKPMLMVLFMRGAFDISDVEMAS
YSLMAYGAGLLNFMLIKVLAPGYYSRQDTKTPVRYGIVAMVSNMVFNIIFAVPFGYVGLA
IATSLSALLNAALLYRGLHLAGVYRLTLPTLVFFGKVAASTLAMGALLWYLLPVVDVWLA
WNIVHRVIALAALIVGGAFLYGTLLLLLGIKPWQMKRARKG