Protein Info for Sama_0790 in Shewanella amazonensis SB2B

Name: valS
Annotation: valyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 transmembrane" amino acids 504 to 527 (24 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 930 (930 residues), 1250.3 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 631 (618 residues), 525.9 bits, see alignment E=1.7e-161 PF09334: tRNA-synt_1g" amino acids 40 to 186 (147 residues), 27.3 bits, see alignment E=4e-10 amino acids 355 to 447 (93 residues), 20.9 bits, see alignment E=3.4e-08 PF13603: tRNA-synt_1_2" amino acids 246 to 312 (67 residues), 34.6 bits, see alignment 3.4e-12 PF08264: Anticodon_1" amino acids 674 to 824 (151 residues), 143.5 bits, see alignment E=1.2e-45 PF10458: Val_tRNA-synt_C" amino acids 884 to 948 (65 residues), 71.9 bits, see alignment 1.1e-23

Best Hits

Swiss-Prot: 72% identical to SYV_ECOLI: Valine--tRNA ligase (valS) from Escherichia coli (strain K12)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 72% identity to cko:CKO_03542)

MetaCyc: 72% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S3P1 at UniProt or InterPro

Protein Sequence (953 amino acids)

>Sama_0790 valyl-tRNA synthetase (RefSeq) (Shewanella amazonensis SB2B)
MEKTYNPQSIEQALYRVWEEKGYFKPHGDASQGNYCIMIPPPNVTGSLHMGHAFQDTIMD
TLIRYQRMKGKNTLWQVGTDHAGIATQMLVERKLEAEQGKSRHDLGRDAFMEKVWEWKAQ
SGGTITSQLRRMGASVDWDRERFTMDEGLSNAVQEVFVRLYDDKLIYRGKRLVNWDPKLH
TAISDLEVENKEKAGHMWHFRYPLAGTELTADGKDYLVVATTRPETMLGDSAVAVHPEDE
RYASLIGKEIILPIVNRRIPIIADEYVDKDFGTGCVKITPAHDFNDYEVGKRHSLPMFNI
LTQDATIRALAEVLNTDGSHNSELDASLPERYAGLDRFKARDAIVAEFETLGLLEKIEPH
ALKVPYGDRSGVVIEPLLTDQWYVAVQKLAQPAIEAVENGDIKFVPQQYENMYFSWMRDI
QDWCISRQLWWGHRIPAWYDEAGKVYVGRSEDEVRQNHNLGSDVKLRQDDDVLDTWFSSA
LWTFSTLGWPEQTPELKTFHPTDVLVTGFDIIFFWVARMIMMTMYFIKDEDGKPQVPFKT
VYVTGLIRDEAGNKMSKSKGNVLDPLDMIDGIDLESLVQKRTGNMMQPQLAAKIEKSTRK
EFENGIEPHGTDALRFTLAAMASTGRDINWDMKRLDGYRSFCNKLWNASRYVLMNTEEQD
CGQGGGDMKLSLADRWVIGKFQETVKAFDEHINAYRFDLAANTLYEFTWNQFCDWYLELT
KPVLQNGSEAEQRGTRHTLVTVLEQLLRLMHPMMPYITETIWDRVKPLAGVEGDTLMLMS
FPEFDAAKVDAKAMADLEWVKQVIVAVRNIRAELNIAPSKPLSALLRGVSDEDKARIEAN
QAFFGTLAKLESMTILAEGEAAPMATTQLIGEMELLIPMAGLIDVAAEMARIDKQLEKLT
GEVARIEGKLSNQGFVAKAPAEVIEKERAKAADIKRDMDKLTEQKAELAKLEA