Protein Info for Sama_0471 in Shewanella amazonensis SB2B

Annotation: ribonuclease G (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 TIGR00757: ribonuclease, Rne/Rng family" amino acids 28 to 439 (412 residues), 551.7 bits, see alignment E=5.7e-170 PF00575: S1" amino acids 52 to 123 (72 residues), 25.9 bits, see alignment E=1.6e-09 PF10150: RNase_E_G" amino acids 138 to 406 (269 residues), 350.8 bits, see alignment E=8.2e-109 PF20833: RNase_E_G_Thio" amino acids 418 to 499 (82 residues), 27.8 bits, see alignment E=3.4e-10

Best Hits

Swiss-Prot: 73% identical to RNG_ECOL6: Ribonuclease G (rng) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K08301, ribonuclease G [EC: 3.1.26.-] (inferred from 100% identity to saz:Sama_0471)

MetaCyc: 73% identical to RNase G (Escherichia coli K-12 substr. MG1655)
Ribonuclease E. [EC: 3.1.26.12]

Predicted SEED Role

"Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-)" in subsystem Bacterial Cell Division or RNA processing and degradation, bacterial (EC 3.1.4.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.12, 3.1.4.-

Use Curated BLAST to search for 3.1.26.- or 3.1.26.12 or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S2S5 at UniProt or InterPro

Protein Sequence (502 amino acids)

>Sama_0471 ribonuclease G (RefSeq) (Shewanella amazonensis SB2B)
MNTVSNSSQKNPRKIGSELLINVTPNEARVALVEHGVLQEVHIERRMKRGLVGNIYKGKI
SRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVADVEKGNFVVRDIAELVRQGQDIMVQ
VVKDPLGTKGARLTTDITLPSRYLVFMPGSAHVGVSQRIESEDERTRLKEATLPFVDEDG
GFIIRTAAEGAGAEELAQDAAFLRRVWAKVAERRKRKGSSLLYQDLALPVRIVRDFVGTD
LDRIQVDSRSTFEELKHFAQEFMPEFEEKIELYSGSVPIFDLYDVENEIQRALGRKVELK
SGGYLIIDQTEAMTTVDINTGAFVGHRNLEETIFNTNLEATQAIARQLRLRNLGGIIIID
FIDMLSEDHRQRVLSSLTSALAHDRVKTNVSGFSGLGLVEMTRKRTRESLEHVLCGECPA
CKGSGYMKTVETVTYEIFREIIRLDKAYDADEFLVYCAPAVHACLSGDEHHHVAELEVYI
GKRIRVQMEPLYAQNKYDVVMV