Protein Info for Sama_0354 in Shewanella amazonensis SB2B

Name: murG
Annotation: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 5 to 354 (350 residues), 362.2 bits, see alignment E=1.4e-112 PF03033: Glyco_transf_28" amino acids 10 to 146 (137 residues), 159.4 bits, see alignment E=1.2e-50 PF13579: Glyco_trans_4_4" amino acids 23 to 142 (120 residues), 33.9 bits, see alignment E=7.8e-12 PF13439: Glyco_transf_4" amino acids 23 to 176 (154 residues), 31.1 bits, see alignment E=4.8e-11 PF04101: Glyco_tran_28_C" amino acids 185 to 350 (166 residues), 133.7 bits, see alignment E=1.3e-42

Best Hits

Swiss-Prot: 100% identical to MURG_SHEAM: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 100% identity to saz:Sama_0354)

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S2F9 at UniProt or InterPro

Protein Sequence (360 amino acids)

>Sama_0354 undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase (RefSeq) (Shewanella amazonensis SB2B)
MGPQTGKRLLVMAGGTGGHVFPALAVARRLASEGWQIRWLGTADRMEARLVPQHGFDIDF
IDIQGVRGNGLLRKLAAPFKVLRSVMQARKVIREFKPDVVLGMGGFASGPGGVAAKLCGI
PLVLHEQNAIPGMTNKLLSRIATRVLCAFEGAFGSLGTTVGNPIREELVALGAKPRESRT
EALKVLVVGGSLGAKVFNDLMPSVTARIAQLQPVTVWHQTGKNNLSTVQAEYQLQGQDGG
VKIAEFIDDMEAAYRWADVVLCRAGALTVSELAAVGLPSILVPYPHAVDDHQTMNARVLV
DAGAAFLVPQPIATTELLADKLQLLAGDRDELTRMGERARAAAVLDATERVAEVCRELAK