Protein Info for Sama_0302 in Shewanella amazonensis SB2B

Annotation: glycoside hydrolase family protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 PF02837: Glyco_hydro_2_N" amino acids 76 to 140 (65 residues), 30.3 bits, see alignment 7.8e-11 PF00703: Glyco_hydro_2" amino acids 209 to 320 (112 residues), 43.2 bits, see alignment E=1.2e-14 PF17786: Mannosidase_ig" amino acids 678 to 767 (90 residues), 75.3 bits, see alignment E=8.9e-25 PF17753: Ig_mannosidase" amino acids 770 to 858 (89 residues), 47.4 bits, see alignment E=2.5e-16

Best Hits

KEGG orthology group: K01192, beta-mannosidase [EC: 3.2.1.25] (inferred from 100% identity to saz:Sama_0302)

Predicted SEED Role

"Beta-mannosidase (EC 3.2.1.25)" in subsystem Mannose Metabolism (EC 3.2.1.25)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S2A7 at UniProt or InterPro

Protein Sequence (863 amino acids)

>Sama_0302 glycoside hydrolase family protein (RefSeq) (Shewanella amazonensis SB2B)
MTTHSFEPFGQSLNSLGQSMDLGGQWQFRQQGKAEWLPAQVPGCNFTDLLANGLIDDPFY
RDNEEKLQWIEQEDWQYRKTFSLDTDWWQNDEVQLVADGLDTYCDLYLNGHKLGDSRNMF
IGQRISCKSLLKVGENLLEIHFRSPIKETLPLHQAAGFTYPAENDKSVEKLSVYNRKAPC
HFGWDWGPRFVTSGIWRAIGLQGIKRGRIDGVHFVQHSLSSDKAEFSFDVSLALCNTDNQ
EGELSLRVACPAAPELNREVPLNRDVPLTEQSALRLDFELTNPNLWWPNGLGEAHLYEFH
FTLLAGDEPLAQHSQAVGLRTLEVINEADGMGQSFYLRVNGQPVFMKGANYIPGDSFIHR
MTPDRHQADFDAVVDANMNMLRVWGGGVYQDEVFYRLADENGILIWQDFMFACSLYPADE
AFLDNVREEAVYNIKRLRNHPCLALWCGNNEVDMGIKHWQWPEKFGYSDELYARLKNDYI
RLFDECLPGAVAELDAGRFYLRSSPIGFWEEDMDHIGNHHYWGVWHGEEPFSEYQKRIPR
FMSEFGFQSFPLASSMARFTEETDWHLESSVLRVHQKHPRGNKLIRSYMEQEYRDPENFP
RLLFLSQVQQAEGLKLAFEAHRAARPFCMGSLYWQLNDTWPAASWSGIDYYGRWKALHYQ
AKRSFRTDLLVVDEVDGAVRVRLVSDRLKSVKARLVLELLDFDGNCLWQEQQALDTPKNT
STQVCQLERASLLGEHNPCQLMLRGRLLDEQGQLLTDCLYYFVPSKDLALKTPALTLEPS
FDAEGLRLTLNTNTLIRQCHLELPEVAGNCSGHCSGNFSDNFFDLVPGEPKCIHIALLGM
DEDTKAALVKGIRCQSLVQEETQ