Protein Info for Sama_0086 in Shewanella amazonensis SB2B
Annotation: coproporphyrinogen III oxidase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to HEMN_ECOLI: Oxygen-independent coproporphyrinogen III oxidase (hemN) from Escherichia coli (strain K12)
KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 100% identity to saz:Sama_0086)MetaCyc: 66% identical to coproporphyrinogen III dehydrogenase (Escherichia coli K-12 substr. MG1655)
HEMN-RXN [EC: 1.3.98.3]
Predicted SEED Role
"Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.99.22)
MetaCyc Pathways
- superpathway of heme b biosynthesis from uroporphyrinogen-III (6/6 steps found)
- heme b biosynthesis II (oxygen-independent) (4/4 steps found)
- 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (3/9 steps found)
- superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.22
Use Curated BLAST to search for 1.3.98.3 or 1.3.99.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1S1P2 at UniProt or InterPro
Protein Sequence (446 amino acids)
>Sama_0086 coproporphyrinogen III oxidase (RefSeq) (Shewanella amazonensis SB2B) MIEKYNYSGPRYTSYPTALEFNDGFTEQDLLSAIEHSKSDKLSLYIHIPFCAKLCYYCGC NKVITRHAHKADQYIEYLASEILNRAPLFKGYTVTQMHWGGGTPTFLSPEQILRLTKLLK DNFNFADEGEFSIEVDPREIELTMLDTLKEAGFNRISIGVQDFNKQVQEAVNRPQDEQFI FDLIGKAKAMGFESTNVDLIYGLPHQTPETFAETIQRILDLSPDRLSVFNYAHLPARFAA QRKIKDEDLPSPQQKLEILHQTIETLVDAGYQYIGMDHFAKPDDELARLQREGKLHRNFQ GYTTQEECDLLGLGVSSISQIGDCYAQNQKDIRPYYEAIDDHGHALWKGCKLNHDDEIRR VVIKQLICHFDLDMAVIDEKFGFTFEDYFAQDLKLLQTFIDDKLVTIENRRIHVSDTGRL LIRNICMCFDVYFREKARQQQFSRVI